Comparison of three enzyme immunoassays to tissue culture for the diagnosis of rotavirus gastroenteritis in infants and young children.
Comparison of three enzyme immunoassays to tissue culture for the diagnosis of rotavirus gastroenteritis in infants and young children.
复制标题
三种酶联免疫分析与组织培养诊断婴幼儿轮状病毒胃肠炎的比较。
DOI：
10.1128/jcm.28.6.1428-1430.1990
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发表时间：
1990
影响因子：
9.4
通讯作者：
Madore,HP
中科院分区：
文献类型：
--
作者：
Christy,C;Vosefski,D;Madore,HP
对Rotazyme II、IDL和Pathfinder三种酶免疫分析法（EIA）检测有症状胃肠炎患儿粪便和直肠拭子标本中的轮状病毒进行了评价，并与原代非洲绿色猴肾细胞中的病毒分离进行了比较。在125份检测标本中，49份组织培养分离轮状病毒阳性;其中40份Rotazyme II阳性，43份IDL阳性，46份Pathfinder EIA阳性。与组织培养分离相比，Rotazyme II、IDL和Pathfinder EIA的敏感性分别为82、88和94%，特异性分别为90、99和95%，总体一致性分别为86、94和94%。
Three enzyme immunoassays (EIAs), Rotazyme II, IDL, and Pathfinder, were evaluated for rotavirus detection in stool and rectal swab specimens from children with symptomatic gastroenteritis and compared with virus isolation in primary African green monkey kidney cells. Of 125 specimens tested, 49 were rotavirus positive by tissue culture isolation; of these 49, 40 were positive by Rotazyme II, 43 were positive by IDL, and 46 were positive by Pathfinder EIAs. As compared with tissue culture isolation, the Rotazyme II, IDL, and Pathfinder EIAs had sensitivities of 82, 88, and 94%, specificities of 90, 99, and 95%, and overall agreements of 86, 94, and 94%, respectively.