Fate of Adipose-Derived Stromal Vascular Fraction Cells after Co-implantation with Fat Grafts: Evidence of Cell Survival and Differentiation in Ischemic Adipose Tissue
Fate of Adipose-Derived Stromal Vascular Fraction Cells after Co-implantation with Fat Grafts: Evidence of Cell Survival and Differentiation in Ischemic Adipose Tissue
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DOI：
10.1097/prs.0b013e31829588b3
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发表时间：
2013-08-01
影响因子：
3.6
通讯作者：
Gao, Yunzhou
中科院分区：
文献类型：
--
作者：
Fu, Su;Luan, Jie;Gao, Yunzhou
背景：一些研究表明，脂肪来源的基质血管部分是脂肪移植物质量改善和长期保留的潜在细胞来源，但明确证明移植基质血管部分细胞的生存和分化潜力是一种动态现象的研究尚未得到广泛报道。方法：分离C57BL/6J-GFP小鼠间质血管部分细胞。将绿色荧光蛋白阳性基质血管部分细胞与C57BL/6J小鼠腹股沟脂肪组织混合后，共植入BALB/c裸鼠体内。采用活体荧光成像技术，对移植的绿色荧光蛋白阳性基质血管部分细胞的存活进行了56天的跟踪观察。采用免疫荧光染色法分析缺血脂肪组织中绿色荧光蛋白阳性基质血管部分细胞在7、14、28、35、42和56天的分化情况。结果：共植入后14天内荧光信号强度急剧下降，此后持续下降，56天时荧光信号强度为17.3%（相对于第1天）。免疫荧光染色显示，部分绿色荧光蛋白阳性细胞从第7天起就能自发分化为脂肪细胞，部分移植基质血管部分细胞可并入新血管。结论：作者提供了令人信服的证据，表明脂肪移植后基质血管部分细胞发生了动态变化。结果证实了移植的间质血管部分细胞能够在脂肪移植物缺血微环境中存活并参与脂肪生成和血管生成过程的原理。
Background: Some studies have suggested that adipose-derived stromal vascular fraction is a potential cell source responsible for the improved quality and long-term retention of fat grafts, but studies that have clearly demonstrated the survival and differentiation potential of the implanted stromal vascular fraction cells as being dynamic phenomena have not been widely reported.Methods: The authors isolated stromal vascular fraction cells from C57BL/6J-GFP mice. Green fluorescence protein-positive stromal vascular fraction cells were mixed with minced inguinal adipose tissue harvested from C57BL/6J mice and then co-implanted into BALB/c nude mice. The survival of implanted green fluorescence protein-positive stromal vascular fraction cells was tracked by in vivo fluorescence imaging for 56 days. Immunofluorescence staining was used to analyze the differentiation of green fluorescence protein-positive stromal vascular fraction cells occurring in ischemic adipose tissue at 7, 14, 28, 35, 42, or 56 days.Results: The fluorescence signal intensity fell drastically within the first 14 days after co-implantation and continued to decrease thereafter, with 17.3 percent signal intensity (relative to day 1) at 56 days. Immunofluorescence staining revealed that some green fluorescence protein-positive cells can spontaneously differentiate into adipocytes from day 7, and some of the implanted stromal vascular fraction cells can incorporate into new blood vessels.Conclusions: The authors show convincing evidence for dynamic changes of stromal vascular fraction cells after co-implantation with fat grafts. The results prove the principle that implanted stromal vascular fraction cells can survive in the ischemic microenvironment of fat grafts and participate in the process of adipogenesis and angiogenesis.