Rhodoferax-related pufM gene cluster dominates the aerobic anoxygenic phototrophic communities in German freshwater lakes.
Rhodoferax-related pufM gene cluster dominates the aerobic anoxygenic phototrophic communities in German freshwater lakes.
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DOI：
10.1111/j.1462-2920.2011.02562.x
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发表时间：
2011-11
影响因子：
5.1
通讯作者：
I. Salka;Zuzana Čuperová;M. Mašín;M. Koblížek;H. Grossart
中科院分区：
文献类型：
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作者：
I. Salka;Zuzana Čuperová;M. Mašín;M. Koblížek;H. Grossart
有氧缺氧光养生物（AAPs）的存在已经在各种海洋环境中被反复报道，但它们在淡水湖中的分布直到最近才被忽视。研究了德国东北部不同营养状态的10个湖泊（贫营养湖泊Stechlin和富腐殖质湖泊Grosse Fuchskuhle）中AAP群落的系统发育组成。AAP群落由α -和β -变形菌组成，但它们的贡献在研究的湖泊中差异很大。结果表明，湖泊AAP群落组成主要受pH和腐殖质含量的影响。碱性湖泊以Betaproteobacteria为主，而富酸性和腐殖质的Lake Grosse Fuchskule西南盆地以Alphaproteobacteria为主（87%）。Betaproteobacteria中最常见的类群是pufM基因簇，与Rhodoferax在系统发育上相关，占所有检索序列的38.5%。在碱性至中性湖泊中，甲变形菌相关序列具有广泛的系统发育多样性，包括6个不同的类群，以鞘单胞菌和rhodobacter-样细菌为主。而在富腐殖质的富酸性湖泊中，甲变形菌相关序列则以AAP群落为主。我们认为，不同的AAP群落结构可能反映了这些细菌在淡水环境中应对不同条件的潜力。
The presence of aerobic anoxygenic phototrophs (AAPs) has been repeatedly reported from various marine environments, but their distribution in freshwater lakes was neglected until recently. We investigated the phylogenetic composition of AAP communities in 10 lakes in Northeastern Germany with different trophic status including oligotrophic Lake Stechlin and humic matter rich Lake Grosse Fuchskuhle. The AAP community was composed by members of Alpha- and Betaproteobacteria, but their contribution varied largely among the studied lakes. Our results show that AAP community composition in the studied lakes was affected mostly by pH and humic matter content. While alkaline lakes were mostly composed of Betaproteobacteria, the acidic and humic matter rich south-west (SW) basin of Lake Grosse Fuchskule was dominated (87%) by Alphaproteobacteria. The most frequent group within Betaproteobacteria was a cluster of pufM genes which was phylogenetically related to Rhodoferax representing 38.5% of all retrieved sequences. Alphaproteobacteria-related sequences had a broader phylogenetic diversity including six different taxa dominated by Sphingomonas- and Rhodobacter-like bacteria in lakes with alkaline to neutral pH. In the acidic and humic matter-rich SW basin of Lake Grosse Fuchskuhle, however, Methylobacterium-related sequences dominated the AAP community. We suggest that the variable AAP community structure might reflect the potential of these bacteria to cope with the contrasting conditions in freshwater environments.