Effects of intestinal colonization by Clostridium difficile and Staphylococcus aureus on microbiota diversity in healthy individuals in China
Effects of intestinal colonization by Clostridium difficile and Staphylococcus aureus on microbiota diversity in healthy individuals in China
复制标题
艰难梭菌和金黄色葡萄球菌肠道定植对中国健康个体微生物群多样性的影响
DOI：
10.1186/s12879-018-3111-z
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发表时间：
2018-05-03
影响因子：
3.7
通讯作者：
Peng, Yibing
中科院分区：
文献类型：
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作者：
Dong, Danfeng;Ni, Qi;Peng, Yibing
背景：肠道致病菌定植是感染的危险因素，并有助于环境污染和疾病传播。肠道微生物群的改变在疾病的发展中也起着关键作用。虽然艰难梭菌和金黄色葡萄球菌是公认的引起医院和社区感染的病原体，但肠道定植尚未得到充分研究。在此，我们探讨了社区健康成年人的总体携带率，并描述了C。梭茵和鼠伤寒沙门方法：收集上海市社区1709名健康志愿者粪便标本，检测是否存在金黄色葡萄球菌。艰难梭菌、甲氧西林敏感S.金黄色葡萄球菌（MSSA）和耐甲氧西林S.金黄色葡萄球菌（MRSA），使用基于培养的技术。为了探索肠道微生物组的差异，使用来自非携带者（CH）、C.结果：共检出12株C. difficile和60 S.金黄色葡萄球菌分离株，分别占总分离株的0.70%和3.51%。8株为MRSA，占13.3%。金黄色葡萄球菌种群。测序结果显示，四个类群的微生物多样性和丰富度相似。然而，在门的水平上，C.与非携带者相比，艰难梭菌或MRSA携带者与拟杆菌门的缺乏和变形菌门的过量相关。在属的水平上，拟杆菌属、普雷沃菌属、粪杆菌属和罗斯拜瑞氏菌属在C.艰难梭菌阳性样品与对照相比，而梭菌XIVa簇物种的比例增加。MRSA携带者中副苏氏菌属和克雷伯菌属的比例较高，但类杆菌属的患病率下降。与MSSA携带者相比，克雷伯菌是MRSA携带者中唯一明显富集的菌属。difficile或S.金黄色葡萄球菌没有显著影响肠道微生物群多样性。然而，C.艰难的携带者可能预示着进一步感染的倾向。我们的研究提供了关于C.梭茵和鼠伤寒沙门金黄色葡萄球菌对健康成年人肠道微生物群组成的定植。
Background:Intestinal colonization by pathogenic bacteria is a risk factor for infection, and contributes to environmental contamination and disease dissemination. Alteration of gut microbiota also plays a pivotal role in the development of disease. Although Clostridium difficile and Staphylococcus aureus are well-recognized pathogens causing nosocomial and community infections, the intestinal colonization was not fully investigated. Herein, we explored their overall carriage rates in healthy adults from the community, and characterized the gut microbiomes of C. difficile and S. aureus carriers.Methods:Fecal samples were collected from 1709 healthy volunteers from communities in Shanghai, China, and tested for the presence of C. difficile, methicillin-sensitive S. aureus (MSSA), and methicillin-resistant S. aureus (MRSA) using culture-based techniques. To explore differences in the gut microbiome, 16S rRNA gene sequencing was conducted using samples from non-carriers (CH), C. difficile carriers (CCD), MRSA carriers (CM), and MSSA carriers (CS).Results:Overall, we detected 12 C. difficile and 60 S. aureus isolates, accounting for 0.70% and 3.51% of total isolates, respectively. Eight isolates were determined to be MRSA, accounting for 13.3% of the S. aureus population. Sequencing data revealed that the microbial diversity and richness were similar among the four groups. However, at the phylum level, carriage of C. difficile or MRSA was associated with a paucity of Bacteroidetes and an overabundance of Proteobacteria compared with non-carriers. At the genus level, the prevalence of the genera Bacteroides, Prevotella, Faecalibacterium, and Roseburia was decreased in C. difficile-positive samples compared with the controls, while the proportion of Clostridium cluster XIVa species was increased. MRSA carriers exhibited a higher proportion of the genera Parasutterella and Klebsiella, but a decreased prevalence of Bacteroides. Compared with MSSA carriers, Klebsiella was the only genus found to be significantly enriched in MRSA carriers.Conclusions:In healthy adults, colonization by C. difficile or S. aureus did not significantly affect gut microbiota diversity. However, the alteration of the gut microbiota composition in C. difficile carriers could indicate a predisposition to further infection. Our study provides essential data on the prevalence and effects of C. difficile and S. aureus colonization on gut microbiota composition in healthy adults.