Magnesium is a critical element for competent development of bovine embryos
Magnesium is a critical element for competent development of bovine embryos
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镁是牛胚胎发育的关键元素
DOI：
10.1016/j.theriogenology.2019.08.015
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发表时间：
2019-12-01
期刊：
影响因子：
2.8
通讯作者：
Du, Fuliang
中科院分区：
文献类型：
--
作者：
An, Liyou;Marjani, Sadie L.;Du, Fuliang
该研究旨在确定镁 (Mg2+) 对牛胚胎发育的影响。我们发现两种市售来源的牛血清白蛋白 (BSA) 和胎牛血清 (FBS) 含有不同量的 Mg2+ 残留物：ICPbio BSA 中为 4 ppm，Sigma BSA 中为 114 ppm，FBS 中为 44 ppm。当使用 CR1 作为基础培养基时，PVA 和 ICPbio BSA 产生的囊胚产量最低 (2.2-2.3%)，而 Sigma BSA 将囊胚产量提高至 18.9% (P < 0.05)。在培养基中添加 1.4 mM MgCl2 可使 ICPbio BSA 组（29.4%）的囊胚率增加，但 PVA 组（5.4%；P < 0.05）的囊胚率并未增加至与 FBS 组相当的水平（33.7%；P> 0.05）。接下来我们发现，培养基中 MgCl2 浓度的增加 (ICPbio BSA) 使囊胚率从 2.6% (0 mM)、38.4% (0.35 mM) 提高到 50.2% (1.4 mM；P < 0.05)，并进一步维持在 44.9% (2.1 mM) 和 43.4% (2.8 mM) (P> 0.05)。然而，当 MgCl2 补充量增加时，囊胚率降低至 31.4% (4.2 mM) 和 29.4% (5.6 mM) (P < 0.05)。当补充 1.4 mM Mg2+（无论其来源如何）时，ICPbio BSA (30.0-33.1%) 和 Sigma BSA (37.4-38.7%) 组均实现了相当的囊胚发育（MgCl2 与 MgSO4；P> 0.05）。在胚胎移植实验中，与共培养的FBS组相比，添加CR1-Mg2+的BSA组的妊娠率（54.3 vs. 41.5%）和产犊率（44.3 vs. 32.5%）分别较高（P < 0.05）。这些结果表明 Mg2+ 是促进有能力的囊胚和足月发育的关键离子。因此，简单高效的成分确定培养基（CR1-Mg2+-BSA）可以成功取代复杂的血清和体细胞共培养。 (C) 2019 Elsevier Inc. 保留所有权利。
The study was designed to determine the impact of magnesium (Mg2+) on bovine embryo development. We found that two commercially available sources of bovine serum albumin (BSA) and fetal bovine serum (FBS) contained different amounts of Mg2+ residue: 4 ppm in ICPbio BSA, 114 ppm in Sigma BSA, and 44 ppm in FBS. When CR1 was used as basal medium, PVA and ICPbio BSA produced the lowest blastocyst yield (2.2-2.3%), whereas Sigma BSA increased blastocyst yield to 18.9% (P < 0.05). Supplementation of 1.4 mM MgCl2 into the medium increased the blastocyst rate in the ICPbio BSA group (29.4%) but not in the PVA group (5.4%; P < 0.05) to a level comparable to that of the FBS group (33.7%; P> 0.05). We next found that increasing concentrations of MgCl2 in the culture medium (ICPbio BSA) elevated blastocyst rate from 2.6% (0 mM), 38.4% (0.35 mM) to 50.2% (1.4 mM; P < 0.05), further maintained at 44.9% (2.1 mM) and 43.4% (2.8 mM) (P> 0.05). However, blastocyst rate was reduced to 31.4% (4.2 mM) and 29.4% (5.6 mM) when MgCl2 supplement was increased (P < 0.05). Comparable blastocyst development was achieved in both ICPbio BSA (30.0-33.1%) and Sigma BSA (37.4-38.7%) groups when 1.4 mM Mg2+ was supplemented regardless of its source (MgCl2 vs. MgSO4; P> 0.05). In embryo transfer experiments, higher rates of pregnancy (54.3 vs. 41.5%) and calving (44.3 vs. 32.5%) were achieved in the CR1-Mg2+-supplemented BSA group compared with the FBS group with co-culture, respectively (P < 0.05). These results demonstrate that Mg2+ is a key ion that promotes competent blastocyst and term development. Therefore, a simple and efficient defined medium (CR1-Mg2+-BSA) can successfully replace complex serum and somatic cell co-culture. (C) 2019 Elsevier Inc. All rights reserved.