A 5' spliced leader is added in trans to both alpha- and beta-tubulin transcripts in Trypanosoma brucei.
A 5' spliced leader is added in trans to both alpha- and beta-tubulin transcripts in Trypanosoma brucei.
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5 剪接前导序列以反式添加到布氏锥虫中的 α 和 β 微管蛋白转录物中。
DOI：
10.1073/pnas.82.17.5695
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发表时间：
1985
影响因子：
11.1
通讯作者：
Agabian,N
中科院分区：
文献类型：
--
作者：
Sather,S;Agabian,N
布鲁氏锥虫大约有15个α -和15个β -微管蛋白基因排列在交替α -和β -微管蛋白基因的串联阵列中。我们研究了来自微管蛋白基因家族的mRNA转录本的结构，并在α和β微管蛋白信息的5‘端发现了一个35核苷酸拼接的前导体（SL），与之前在变异表面糖蛋白（VSG） mRNA的5’端发现的前导体相同。微管蛋白基因间区未编码35-核苷酸SL序列；相反，SL序列显然起源于与微管蛋白基因不相连的位点编码的小RNA转录本。微管蛋白基因的串联排列以及在α -和β -微管蛋白转录本的5'端存在的SL确定了RNA在SL和微管蛋白mRNA之间的连接发生在反式中。
The approximately 15 alpha- and 15 beta-tubulin genes of Trypanosoma brucei are arranged in a tandem array of alternating alpha- and beta-tubulin genes. We have examined the structure of mRNA transcripts from the tubulin gene family and have found at the 5' ends of both alpha- and beta-tubulin messages a 35-nucleotide spliced leader (SL) identical to that identified previously on the 5' ends of variant surface glycoprotein (VSG) mRNAs. No 35-nucleotide SL sequences were encoded in the tubulin intergenic regions; instead, the SL sequence apparently originates from small RNA transcripts encoded at sites that are unlinked to the tubulin genes. The tandem arrangement of the tubulin genes and the presence of the SL at the 5' end of both alpha- and beta-tubulin transcripts establishes that RNA joining between the SL and tubulin mRNA occurs in trans.