Speed quantification and tracking of moving objects in adaptive optics scanning laser ophthalmoscopy.
Speed quantification and tracking of moving objects in adaptive optics scanning laser ophthalmoscopy.
复制标题
自适应光学扫描激光检眼镜中移动物体的速度量化和跟踪。
DOI：
10.1117/1.3548880
复制
发表时间：
2011
影响因子：
3.5
通讯作者：
Roorda,Austin
中科院分区：
文献类型：
--
作者：
Tam,Johnny;Roorda,Austin
使用自适应光学扫描激光检眼镜（AOSLO）可以无创地解析人类视网膜的微观特征。我们描述了一种改进的方法来跟踪和量化 AOSLO 视频中移动物体的速度，这对于表征视网膜毛细血管的血流动力学是必要的。在视频采集期间，感兴趣的对象相对于背景组织不断运动（对象运动）。由于视频录制期间的连续眼球运动（眼球运动），背景组织相对于 AOSLO 不断运动。 AOSLO 采集数据的位置也在连续运动，因为成像源以光栅扫描方式扫过视网膜（光栅扫描）。我们表明，考虑物体运动、眼睛运动和光栅扫描的结合对于准确量化物体速度非常重要。所提出的方法在实验 AOSLO 视频以及虚拟 AOSLO 生成的合成视频上均表现良好。这些方法提高了使用 AOSLO 成像研究血流动力学方法的准确性。
Microscopic features of the human retina can be resolved noninvasively using an adaptive optics scanning laser ophthalmoscope (AOSLO). We describe an improved method to track and quantify the speed of moving objects in AOSLO videos, which is necessary for characterizing the hemodynamics of retinal capillaries. During video acquisition, the objects of interest are in constant motion relative to the background tissue (object motion). The background tissue is in constant motion relative to the AOSLO, due to continuous eye motion during video recordings (eye motion). The location at which AOSLO acquires data is also in continuous motion, since the imaging source is swept in a raster scan across the retina (raster scanning). We show that it is important to take into consideration the combination of object motion, eye motion, and raster scanning for accurate quantification of object speeds. The proposed methods performed well on both experimental AOSLO videos as well as synthetic videos generated by a virtual AOSLO. These methods improve the accuracy of methods to investigate hemodynamics using AOSLO imaging.