Changes in expression of growth-associated protein-43 in trigeminal ganglion neurons and of the jaw opening reflex following inferior alveolar nerve transection in rats.
Changes in expression of growth-associated protein-43 in trigeminal ganglion neurons and of the jaw opening reflex following inferior alveolar nerve transection in rats.
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DOI：
10.1111/eos.12021
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发表时间：
2013-04
影响因子：
1.9
通讯作者：
Kohei Teramoto;Y. Tsuboi;M. Shinoda;S. Hitomi;K. Abe;K. Kaji;Takaaki Tamagawa;Azumi Suzuki;N. Noma;Masayuki Kobayashi;O. Komiyama;K. Urata;K. Iwata
中科院分区：
文献类型：
--
作者：
Kohei Teramoto;Y. Tsuboi;M. Shinoda;S. Hitomi;K. Abe;K. Kaji;Takaaki Tamagawa;Azumi Suzuki;N. Noma;Masayuki Kobayashi;O. Komiyama;K. Urata;K. Iwata
本研究的目的是阐明生长相关蛋白-43（GAP-43）参与下牙槽神经切断（IANX）后初级传入三叉神经节（TG）神经元的再生。IANX后3d，大鼠海马GAP-43免疫反应阳性TG神经元的数量明显多于Sham组。生长相关蛋白-43 IR TG神经元在IANX后30天也被检测到，并且GAP-43 IR TG神经元的数量在IANX模型中显著更高，直到第30天。IANX后30 d，大（>600 μm2）GAP-43 IR TG神经元的相对数量显著低于0 d，而小（<400 μm2）GAP-43 IR TG神经元的相对数量显著高于0 d。为了评价受损IAN的功能恢复，在IANX前后测量由IAN的电刺激引起的下颌张开反射（JOR）。在30天的研究过程中，开颌反射的发生率逐渐增加，电刺激诱发JOR的相对阈值逐渐降低。在IANX后30天，JOR发生率和相对JOR阈值与假手术大鼠相似。本研究结果表明，GAP-43在TG神经元的表达变化后IANX参与再生和功能恢复的横断IAN。
The aim of the present study was to clarify an involvement of growth-associated protein-43 (GAP-43) in the regeneration of primary afferent trigeminal ganglion (TG) neurons following inferior alveolar nerve transection (IANX). A larger number of GAP-43 immunoreactive (GAP-43 IR) TG neurons was observed in rats 3 d after IANX compared with sham rats. Growth-associated protein-43 IR TG neurons were also detected for 30 d after IANX, and the number of GAP-43 IR TG neurons was significantly higher in the IANX model until day 30. The relative number of large (>600 μm2) GAP-43 IR TG neurons was significantly lower, whereas the relative number of small (<400 μm2) GAP-43 IR TG neurons was significantly higher than that at day 0 until 30 d after IANX. To evaluate the functional recovery of damaged IAN, the jaw opening reflex (JOR), elicited by the electrical stimulation of the IAN, was measured before and after IANX. Jaw opening reflex occurrence was gradually increased and the relative threshold of electrical stimulation eliciting JOR was gradually decreased over the 30-d duration of the study. On day 30 after IANX, the JOR occurrence and relative JOR threshold were similar to those in sham rats. The present findings suggest that changes in the expression of GAP-43 in TG neurons after IANX are involved in regeneration and functional recovery of the transected IAN.