Metabolism of specifically labelled pyruvate by mouse embryos during culture from the two-cell stage to the blastocyst.
Metabolism of specifically labelled pyruvate by mouse embryos during culture from the two-cell stage to the blastocyst.
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从两细胞阶段到囊胚培养期间，小鼠胚胎对特异性标记的丙酮酸的代谢。
DOI：
10.1071/bi9700877
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发表时间：
1970
期刊：
影响因子：
--
通讯作者：
D. Whittingham
中科院分区：
文献类型：
--
作者：
R. Wales;D. Whittingham
将双细胞小鼠胚胎在含有0·5mM [1_14C]丙酮酸钠或[2_14C]丙酮酸钠的培养基中培养24、48和72小时。当使用[1_14C]丙酮酸时，放射性乳酸是在培养基中积累的主要代谢物。当[2_14C]丙酮酸盐为能量来源时，在培养期结束时，在培养基中鉴别标记的乳酸盐和乙酸盐。
Two-cell mouse embryos were cultured for 24, 48, and 72 hr in media containing 0·5 mM sodium [l_14C]pyruvate or sodium [2_14C]pyruvate. Radioactive lactate was the main metabolite accumulating in the medium when [1_14C]pyruvate was used. Labelled lactate and acetate were identified in the medium at the end of the culture period when [2_14C]pyruvate was the energy source.