The splenic marginal zone is absent in alymphoplastic aly mutant mice
The splenic marginal zone is absent in alymphoplastic aly mutant mice
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DOI：
10.1002/eji.1830260324
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发表时间：
1996-03-01
影响因子：
5.4
通讯作者：
Miyasaka, M
中科院分区：
文献类型：
--
作者：
Koike, R;Nishimura, T;Miyasaka, M
aly是小鼠中独特的自发性常染色体隐性突变，其导致全身淋巴结（LN）和派伊尔集合淋巴结（PP）的缺陷。Aly还诱导脾脏中的异常组织学发现以及体液和细胞介导的免疫功能的缺乏，尽管Aly/Aly小鼠的淋巴细胞在体外显示出实际上正常的免疫应答。我们用免疫组织化学和电子显微镜详细研究了aly小鼠脾脏的结构。发现aly/aly小鼠脾脏边缘区（MZ）的边缘嗜金属粒细胞（MM）、边缘区巨噬细胞（MZM）和成纤维网状细胞（FRC）的特异性抗原表达不足。形态学分析表明，aly/aly小鼠脾脏MZ结构缺陷，这可能是导致aly/aly小鼠严重免疫缺陷的部分原因。然后，我们进行了正常和aly小鼠之间的相互骨髓移植实验（BMT），发现LN的形成和脾MZ的发展在这些小鼠之间有明确的相关性;即LN的成功发展总是与MZ结构的出现相关，而LN发展的失败总是与MZ的发展的缺乏相关。这些结果表明，一个共同的因素可能调节LN和MZ的脾的产生。
aly is a unique spontaneous autosomal recessive mutation in mice that causes a deficiency in the systemic lymph nodes (LN) and Peyer's patches (PP). aly also induces abnormal histological findings in the spleen and a deficiency in humoral and cell-mediated immune functions, although lymphocytes of the aly/aly mouse show virtually normal immune responses in vitro. We studied the structure of the spleen of aly mice in detail by immunohistochemistry and electron microscopy. We fond that the spleen of the aly/aly mouse was deficient in the expression of specific antigens for marginal metallophils (MM), marginal zone macrophages (MZM) and fibroblastic reticular cells (FRC), which are components of the marginal zone (MZ) of the spleen. Morphological analysis indicated that the aly/aly spleen is deficient in the structure of MZ, which may, in part, account for the severe immunodeficiency in the aly/aly mouse. We then performed reciprocal bone marrow transplantation experiments (BMT) between normal and aly mice and found a clear correlation between the formation of LN and the development of the splenic MZ in these mice; i.e. successful development of LN was invariably associated with the appearance of the MZ structure, whereas the failure of LN development was always associated with the absence of the development of MZ. These BMT results suggest that a common factor may regulate the generation of both LN and MZ of the spleen.