Specific expression of annexin III in rat-small-hepatocytes
Specific expression of annexin III in rat-small-hepatocytes
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DOI：
10.1016/s0006-291x(02)02911-x
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发表时间：
2003-01-17
影响因子：
3.1
通讯作者：
Hayakawa, T
中科院分区：
文献类型：
--
作者：
Niimi, S;Oshizawa, T;Hayakawa, T
小肝细胞是表现出高生长潜能和分化能力等特征表型的细胞。为了鉴定大鼠小肝细胞特异的蛋白质，我们用双向凝胶电泳法分离了大鼠小肝细胞和实质肝细胞中的细胞蛋白。比较它们的图谱发现，小肝细胞中有一种分子质量为37 kDa的蛋白质，而实质肝细胞中没有这种蛋白质。用蛋白水解肽质量指纹图谱鉴定该蛋白为膜联蛋白III。用抗Annexin III抗体进行Western印迹分析，证实了鉴定的有效性。(C)2002年埃尔塞维尔科学公司(美国)。版权所有。
Small hepatocytes are cells that express characteristic phenotypes such as a high growth potential and differentiation capacity. In order to identify rat-small-hepatocyte specific proteins, we separated the cellular protein: of isolated small and parenchymal hepatocytes by 2D polyacrylamide gel electrophoresis. Comparison of their profiles revealed a protein with a molecular mass of 37 kDa in the small hepatocytes that was not present in the parenchymal hepatocytes. Proteolytic peptide mass fingerprinting was used to identify the protein and it was found to be annexin III. The validity of the identification was confirmed by Western blot analysis with anti-annexin III antibody. (C) 2002 Elsevier Science (USA). All rights reserved.