Plo1 kinase recruitment to the spindle pole body and its role in cell division in Schizosaccharomyces pombe
Plo1 kinase recruitment to the spindle pole body and its role in cell division in Schizosaccharomyces pombe
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DOI：
10.1091/mbc.10.8.2771
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发表时间：
1999-08-01
影响因子：
3.3
通讯作者：
Hagan, IM
中科院分区：
文献类型：
--
作者：
Mulvihill, DP;Petersen, J;Hagan, IM
波罗激酶在细胞分裂过程中发挥多种作用。需要分裂酵母波罗相关激酶Plo 1来组装有丝分裂纺锤体，有丝分裂完成后预测胞质分裂和分隔位点的前期肌动蛋白环（Ohkura等人，1995; Bahler等人，1998年）。我们发现，Plo 1与有丝分裂，但不间期纺锤体极体（SPB）。SPB协会Plo 1是最早的裂变酵母有丝分裂事件记录的日期。从有丝分裂定型到后期早期B，SPB的关联是强的，之后Plo 1信号变得非常弱，并最终在纺锤体破裂时消失。SPB协会的Plo 1需要有丝分裂促进因子（MPF）的活动，而其解体需要的后期促进复合物的活动。stf 1.1突变绕过了对MPF激活剂Cdc 25的通常需要（哈德逊et al.，1990年）。值得注意的是，Plo 1与stf 1.1细胞的间期SPB不适当地关联。这些数据是一致的新兴的主题，从许多系统，波罗激酶参与调节MPF，以确定有丝分裂的承诺的时间，并可能表明，极点协会是一个关键方面的Plo 1功能。Plo 1不与SPB时，分隔是不适当的驱动Spg 1途径的失调，并保持SPB相关，如果分隔发生在一个纺锤体的存在。因此，既不需要Plo 1募集到SPB，也不需要其离开SPB进行分隔;然而，plo 1（+）的过表达激活Spg 1通路，并导致短暂的Cdc 7募集到SPB和多轮分隔。
Polo kinases execute multiple roles during cell division. The fission yeast polo related kinase Plo1 is required to assemble the mitotic spindle, the prophase actin ring that predicts the site for cytokinesis and for septation after the completion of mitosis (Ohkura et al., 1995; Bahler et al., 1998). We show that Plo1 associates with the mitotic but not interphase spindle pole body (SPB). SPB association of Plo1 is the earliest fission yeast mitotic event recorded to date. SPB association is strong from mitotic commitment to early anaphase B, after which the Plo1 signal becomes very weak and finally disappears upon spindle breakdown. SPB association of Plo1 requires mitosis-promoting factor (MPF) activity, whereas its disassociation requires the activity of the anaphase-promoting complex. The stf1.1 mutation bypasses the usual requirement for the MPF activator Cdc25 (Hudson et al., 1990). Significantly, Plo1 associates inappropriately with the interphase SPB of stf1.1 cells. These data are consistent with the emerging theme from many systems that polo kinases participate in the regulation of MPF to determine the timing of commitment to mitosis and may indicate that pole association is a key aspect of Plo1 function. Plo1 does not associate with the SPB when septation is inappropriately driven by deregulation of the Spg1 pathway and remains SPB associated if septation occurs in the presence of a spindle. Thus, neither Plo1 recruitment to nor its departure from the SPB are required for septation; however, overexpression of plo1(+) activates the Spg1 pathway and causes transient Cdc7 recruitment to the SPB and multiple rounds of septation.