Complex II inactivation is lethal in the nematode Caenorhabditis elegans
Complex II inactivation is lethal in the nematode Caenorhabditis elegans
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DOI：
10.1016/s1567-7249(02)00069-7
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发表时间：
2002-12-01
期刊：
影响因子：
4.4
通讯作者：
Ishii, N
中科院分区：
文献类型：
--
作者：
Ichimiya, H;Huet, RG;Ishii, N
采用RNA介导的干扰（RNAi）技术系统性地使线粒体电子传递链中复合物II的四个亚基失活。当使用浸泡法使RNA失活时，使三个亚基（ceSDHB、ceSDHC和ceSDHD）失活的主要结果是胚胎致死。采用喂食法将来自第四个亚基（ceSDHA）的双链RNA传递给野生型、mev - 1（复合物II的ceSDHC发生突变）和gas - 1动物（复合物I的一个基因发生突变）。仅在mev - 1遗传背景下，且以氧依赖的方式，存活率降低。总之，这些数据进一步证明复合物II完整性受损可导致对氧化应激的敏感性。（C）2002年爱思唯尔科学出版社和线粒体研究学会。版权所有。
RNA-mediated interference (RNAi) was employed to systematically inactivate the four subunits of complex II in the mitochondrial electron transport chain. Embryonic lethality was the predominant result of inactivating three subunits (ceSDHB, ceSDHC, and ceSDHD) when using the soaking method to inactivate RNA. The feeding method was employed to deliver dsRNA from the fourth subunit (ceSDHA) to wild-type, mev-1 (mutated in ceSDHC of complex II), and gas-1 animals (mutated in a complex I gene). Survival was reduced only in the mev-1 genetic background, and in an oxygen-dependent fashion. Collectively, these data provide further evidence that compromised complex II integrity can result in sensitivity to oxidative stress. (C) 2002 Elsevier Science B.V. and Mitochondria Research Society. All rights reserved.