COMPARISON OF THE CHROMOSOMES OF TRITICUM-TIMOPHEEVI WITH RELATED WHEATS USING THE TECHNIQUES OF C-BANDING AND INSITU HYBRIDIZATION

COMPARISON OF THE CHROMOSOMES OF TRITICUM-TIMOPHEEVI WITH RELATED WHEATS USING THE TECHNIQUES OF C-BANDING AND INSITU HYBRIDIZATION
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DOI:
10.1007/bf00303647
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发表时间:
1982-01-01
影响因子:
5.4
通讯作者:
SHEPHERD, KW
SHEPHERD, KW
中科院分区:
农林科学1区
文献类型:
--
作者:
HUTCHINSON, J;MILLER, TE;SHEPHERD, KW

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四倍体小麦T. timopheevi (Genome AAGG)和T. araraticum (Genome AAGG)在有丝分裂时染色体呈c带。首先与基因组公式为AAAAGG的六倍体种T. zhukovskyi进行比较,确定了带状和非带状染色体的身份。用c带法研究了小麦与小麦F1杂种的减数分裂配对。带状染色体属于g基因组,非带状染色体属于A基因组。2对伴体染色体中只有1对具有强异色带。利用c带和克隆核糖体RNA基因探针原位杂交技术,对timopheevi和T. dicoccum基因组(Genome AABB)之间的关系进行了评估。不同种属间异染色质数量和分布存在差异,易位也存在差异。
The chromosomes of the tetraploid wheats T. timopheevi (Genome AAGG) and T. araraticum (Genome AAGG) were C-banded at mitosis. The identity of the banded and unbanded chromosomes was then established by first making comparisons with the hexaploid species T. zhukovskyi which has the genome formula AAAAGG. The meiotic pairing in F1 hybrids between T. timopheevi and diploid wheats was examined by means of C-banding. The banded chromosomes belonged to the G. genome, while the unbanded chromosomes belonged to the A genome. Only one of the 2 pairs of satellited chromosomes had strong heterochromatic bands. The relationship between the genomes of T. timopheevi and T. dicoccum (Genome AABB) was then assessed at meiosis in hybrids between these species, using the techniques of C-banding and in situ hybridization of a cloned ribosomal RNA gene probe. There were differences both in the amount and distribution of heterochromatin and also translocation differences between the species.