Time-course alteration of lipoxygenase activity in blast-infected rice leaves
Time-course alteration of lipoxygenase activity in blast-infected rice leaves
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感染稻瘟病的水稻叶片中脂氧合酶活性的时程变化
DOI：
10.3186/jjphytopath.56.26
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
J. Togashi
中科院分区：
文献类型：
--
作者：
T. Namai;Tadahiro Kato;Y. Yamaguchi;J. Togashi
为了揭示水稻对稻瘟病的抗性与从水稻中分离出的作为抗稻瘟病物质的含氧C-18不饱和脂肪酸的量之间的关系，研究了水稻叶片中参与这些化合物合成的脂氧合酶（LOX）活性的变化。虽然水稻叶片中的LOX活性对喷水敏感而略有增加，但稻瘟病感染后其活性明显大幅增强。接种后几天内LOX活性的变化模式取决于水稻品种和稻瘟菌品种的组合。在表现出不亲和反应的水稻叶片中，LOX活性从喷雾接种第一天开始迅速升高，两三天后达到最大，随后下降。在相容组合中，接种后 7 天观察到最高活性，尽管 LOX 活性在两三天内暂时上升。田间抗性水平较高的水稻品种比低水平的水稻品种表现出更高的LOX活性。此外，所有受检品种的成熟叶中的LOX活性也高于刚刚展开的幼叶。
To reveal the relationship between the resistance to rice blast disease on rice plants and the amount of oxygenated C-18 unsaturated fatty acids isolated from rice plant as anti-blast substances, the change of the activity of lipoxygenase (LOX) which participates in the synthesis of these compounds in rice leaves was investigated. Although LOX activity in rice leaves was sensitive to the water-spraying resulted in a little increment, it has been clearly and largely enhanced by blast infection. The pattern of change in LOX activity with days after inoculation depended on the combination of rice cultivar and the race of rice blast fungus. In rice leaves shown incompatible reaction, LOX activity increased rapidly from the first day of spray-inoculation and reached to the maximum two or three days after, and declined thereafter. In the compatible combination, the highest activity was observed seven days after inoculation, although LOX activity rose temporarily in two or three days. Rice cultivars having higher level of field resistance showed higher LOX activity than that with low level of it. In addition, LOX activity in mature leaves was also greater than that of the just expanded young leaves of all cultivars examined.