Endothelial and perivascular cells maintain haematopoietic stem cells.
Endothelial and perivascular cells maintain haematopoietic stem cells.
复制标题
DOI：
10.1038/nature10783
复制
发表时间：
2012-01-25
期刊：
影响因子：
64.8
通讯作者：
Morrison, Sean J.
中科院分区：
文献类型：
--
作者：
Ding, Lei;Saunders, Thomas L.;Enikolopov, Grigori;Morrison, Sean J.
已经提出了多种细胞类型来创建造血干细胞（HSC）的小生境。然而，HSC维持因子的表达模式尚未得到系统的研究，也没有这样的因子从任何候选的小生境细胞中被有条件地删除。因此，这些因素的细胞来源是不确定的。干细胞因子（SCF）是维持HSC的关键生态位组分。使用Scfgfp敲入小鼠，我们发现Scf主要由整个骨髓的血管周围细胞表达。造血细胞、成骨细胞、Nestin-Cre或Nestin-CreER表达细胞中Scf有条件缺失时，HSC频率和功能不受影响。然而，当从内皮细胞或表达瘦素受体（Lepr）的血管周围基质细胞中删除Scf时，骨髓中的HSC被耗尽。当Scf从内皮细胞和表达Lepr的血管周围细胞中删除时，大多数HSC丢失。HSC存在于血管周围的小生境中，其中多种细胞类型表达促进HSC维持的因子。
Multiple cell types have been proposed to create niches for haematopoietic stem cells (HSCs). However, the expression patterns of HSC maintenance factors have not been systematically studied and no such factor has been conditionally deleted from any candidate niche cell. Thus, the cellular sources of these factors are undetermined. Stem Cell Factor (SCF) is a key niche component that maintains HSCs. Using Scfgfp knock-in mice we found Scf was primarily expressed by perivascular cells throughout bone marrow. HSC frequency and function were not affected when Scf was conditionally deleted from haematopoietic cells, osteoblasts, Nestin-Cre, or Nestin-CreER-expressing cells. However, HSCs were depleted from bone marrow when Scf was deleted from endothelial cells or Leptin receptor (Lepr)-expressing perivascular stromal cells. Most HSCs were lost when Scf was deleted from both endothelial and Lepr-expressing perivascular cells. HSCs reside in a perivascular niche in which multiple cell types express factors that promote HSC maintenance.
登录
查看更多内容
影响因子：
23.9
作者：
Butler JM;Nolan DJ;Vertes EL;Varnum-Finney B;Kobayashi H;Hooper AT;Seandel M;Shido K;White IA;Kobayashi M;Witte L;May C;Shawber C;Kimura Y;Kitajewski J;Rosenwaks Z;Bernstein ID;Rafii S
通讯作者：
Rafii S
影响因子：
64.5
作者：
Kiel, MJ;Yilmaz, ÖH;Morrison, SJ
通讯作者：
Morrison, SJ
影响因子：
0.7
作者：
Liu, F;Woitge, HW;Kream, BE
通讯作者：
Kream, BE
影响因子：
82.9
作者：
Kollet, Orit;Dar, Ayelet;Lapidot, Tsvee
通讯作者：
Lapidot, Tsvee
影响因子：
5.3
作者：
Balordi, Francesca;Fishell, Gord
通讯作者：
Fishell, Gord