Validation of a rapid measure of blood PUFA levels in humans
Validation of a rapid measure of blood PUFA levels in humans
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DOI：
10.1007/s11745-007-3140-7
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发表时间：
2008-02-01
期刊：
影响因子：
1.9
通讯作者：
Ryan, Alan S.
中科院分区：
文献类型：
--
作者：
Bailey-Hall, Eileen;Nelson, Edward B.;Ryan, Alan S.
开发了一种涉及指尖采血和滤纸血液点样的检测方法，用于测定血液中多不饱和脂肪酸 (PUFA) 的水平。将来自指尖采血的毛细管全血吸干到抗氧化剂浸渍的滤纸上，风干、皂化并使用氢氧化钠和三氟化硼的甲醇溶液进行甲基化。该方法与之前描述的方法不同，因为不需要分离血浆和红细胞（RBC），不需要薄层色谱（TLC）来分离磷脂，不需要在酯交换之前初始提取脂质，并且脂肪酸甲酯（FAME）方法能够甲基化甾醇酯、游离脂肪酸和鞘磷脂。二十六名受试者通过指尖采血和静脉穿刺提供了血液样本。毛细血管全血中的长链多不饱和脂肪酸 (LC-PUFA) 水平与静脉血中红细胞和 PL 的水平相关（P < 0.001，R-2 范围为 0.64 至 0.86）。尽管非常显着（P < 0.002），但毛细血管全血中花生四烯酸 (ARA) 水平与红细胞和血浆磷脂 (PL) 中 ARA 水平之间相关性的 R-2 值相对较低（R-2 分别 = 0.31-0.41）。结果表明，所描述的指尖刺血测定是测量特定 LC-PUFA 水平的快速、可靠的方法。
An assay involving a finger stick and filter paper blood spotting was developed to determine polyunsaturated fatty acid (PUFA) levels in blood. Capillary whole blood from a finger stick was blotted on antioxidant impregnated filter paper, air dried, saponified and methylated using sodium hydroxide and boron trifluoride in methanol. The method differed from those described previously because separation of plasma and red blood cells (RBCs) was not needed, thin-layer chromatography (TLC) was not required to separate phospholipids, initial extraction of lipids before transesterification was not necessary, and the fatty acid methyl ester (FAME) method was able to methylate steryl esters, free fatty acids, and sphingomyelins. Twenty-six subjects provided blood samples by finger stick and venipuncture. Levels of long-chain polyunsaturated fatty acids (LC-PUFA) from capillary whole blood were correlated with those from RBCs and PLs in venous blood (P < 0.001, R-2 ranged from 0.64 to 0.86). Although highly significant (P < 0.002), the R-2 values for the correlation between arachidonic acid (ARA) levels in capillary whole blood with ARA levels in RBCs and plasma phospholipids (PLs) were relatively lower (R-2 = 0.31-0.41, respectively). Results indicate that the described finger stick assay represents a fast, reliable method to measure specific LC-PUFA levels.