Mapping and quantitative trait loci analysis of verticillium wilt resistance genes in cotton
Mapping and quantitative trait loci analysis of verticillium wilt resistance genes in cotton
复制标题
棉花黄萎病抗性基因定位及数量性状位点分析
DOI：
10.1111/j.1744-7909.2007.00612.x
复制
发表时间：
2008-02-01
影响因子：
11.4
通讯作者：
Li, Yun-Hai
中科院分区：
文献类型：
--
作者：
Wang, Hong-Mei;Lin, Zhong-Xu;Li, Yun-Hai
棉花黄萎病是世界上几乎所有产棉国棉花生产的最严重的限制因素之一。本研究以感病品种“新陆早1号”（XLZ 1）为试材，对其进行了田间试验。抗感品系“海7124”（H7124）。以海岛棉、海岛棉及其F-2：3家系为材料，对海岛棉黄萎病的病情指数进行了定位研究。共将430个SSR标记定位在41个连锁群中，图谱总长度为3745.9cM，平均位点间距离为8.71cM。根据2004年7月22日和8月24日的病情调查结果，分别检测到4个和5个数量性状位点。这9个QTL对表型变异的解释率为10.63-28.83%，其中6个位于D亚基因组上。位于同一标记区间的两个QTL可以部分解释两个性状间的显著相关。本研究筛选出了可解释棉花抗病性大变异的QTL，为棉花抗病育种提供了重要的理论依据。
Verticillium wilt is one of the most serious constraints to cotton production in almost all of the cotton-growing countries. In this study, "XinLuZao1" (XLZ1), a susceptible cultivar Gossypium hirsutum L. and "Hai7124" (H7124), a resistant line G. barbadense, and their F-2:3 families were used to map and study the disease index induced by verticillium wilt. A total of 430 SSR loci were mapped into 41 linkage groups; the map spanned 3 745.9 cM and the average distance between adjacent loci was 8.71 cM. Four and five quantitative trait loci (QTLs) were detected based on the disease index investigated on July 22 and August 24 in 2004, respectively. These nine QTLs explained 10.63-28.83% of the phenotypic variance, six of them were located on the D sub-genome. Two QTLs located in the same marker intervals may partly explain the significant correlation of the two traits. QTLs explaining large phenotypic variation were identified in this study, which may be quite useful in cotton anti-disease breeding.