Increase in transcript accumulation of Psy1 and e-Lcy genes in grain development is associated with differences in seed carotenoid content between durum wheat and tritordeum
Increase in transcript accumulation of Psy1 and e-Lcy genes in grain development is associated with differences in seed carotenoid content between durum wheat and tritordeum
复制标题
DOI：
10.1007/s11103-013-0160-y
复制
发表时间：
2014-04
影响因子：
5.1
通讯作者：
C. Rodríguez-Suárez;Elena Mellado-Ortega;D. Hornero-Méndez;S. G. Atienza
中科院分区：
文献类型：
--
作者：
C. Rodríguez-Suárez;Elena Mellado-Ortega;D. Hornero-Méndez;S. G. Atienza
富含类胡萝卜素的饮食与某些疾病的低风险有关。谷物在人类饮食中的重要性已经将育种计划引向类胡萝卜素增强，以减轻发展中国家的这些缺乏，并在发达国家提供新的功能性食品。从硬粒小麦（Triticum turgidumssp.）硬粒小麦（Durum）和野生大麦（Hordeum chilense）天然呈现比硬粒小麦高5-8倍的类胡萝卜素水平。作为一种新的功能性食品的tritordeum性质的改善需要澄清的生物合成步骤，类胡萝卜素积累的种子，不同于硬粒小麦。在这项工作中，类异戊二烯和类胡萝卜素生物合成途径的9个基因的表达模式进行了监测，在硬质小麦和tritordeum的籽粒发育。此外，在谷物发育过程中和成熟种子中的色素（叶绿素和类胡萝卜素）的精细鉴定和定量已经得到解决。Psy 1、Psy 2、Zds、e-Lcy和b-Lcy的转录水平与成熟籽粒中的类胡萝卜素含量相关。同源异型基因Psy 1、e-Lcy从H. chilense和高叶黄素酯化发现tritordeum可能有助于解释与硬粒小麦类胡萝卜素积累的差异。
Carotenoid rich diets have been associated with lower risk of certain diseases. The great importance of cereals in human diet has directed breeding programs towards carotenoid enhancement to alleviate these deficiencies in developing countries and to offer new functional foods in the developed ones. The new cereal tritordeum (×TritordeumAscherson et Graebener) derived from durum wheat (Triticum turgidumssp. durum) and the wild barleyHordeum chilense, naturally presents carotenoid levels 5–8 times higher than those of durum wheat. The improvement of tritordeum properties as a new functional food requires the elucidation of biosynthetic steps for carotenoid accumulation in seeds that differ from durum wheat. In this work expression patterns of nine genes from the isoprenoid and carotenoid biosynthetic pathways were monitored during grain development in durum wheat and tritordeum. Additionally, a fine identification and quantification of pigments (chlorophylls and carotenoids) during grain development and in mature seeds has been addressed. Transcript levels ofPsy1,Psy2,Zds,e-Lcyandb-Lcywere found to correlate to carotenoid content in mature grains. The specific activation of the homeologous genesPsy1,e-LcyfromH. chilenseand the high lutein esterification found in tritordeum may serve to explain the differences with durum wheat in carotenoid accumulation.