Recovery of cell nuclei from 15,000 years old mammoth tissues and its injection into mouse enucleated matured oocytes
Recovery of cell nuclei from 15,000 years old mammoth tissues and its injection into mouse enucleated matured oocytes
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从 15,000 年前的猛犸象组织中回收细胞核并将其注射到小鼠去核成熟卵母细胞中
DOI：
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发表时间：
2009
期刊：
影响因子：
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通讯作者：
A. Iritani
中科院分区：
文献类型：
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作者：
H. Kato;M. Anzai;T. Mitani;M. Morita;Yui Nishiyama;A. Nakao;K. Kondo;P. A. Lazarev;T. Ohtani;Y. Shibata;A. Iritani
在这里，我们报道了从14000 - 15000年前的猛犸组织中恢复细胞核，并通过体细胞核移植（SCNT）将这些细胞核注射到小鼠去核的成熟卵母细胞中。从皮肤和肌肉组织中，成功地恢复了细胞核样结构。然后将这些细胞核注射到去核的卵母细胞中，超过一半的卵母细胞能够存活。注射后的细胞核未被分解，仍保持原有的核结构。卵母细胞在注射后1小时未出现核膜消失或染色体过早凝结（PCC），注射后7小时未形成原核样结构。经冻融小鼠骨髓细胞核注入的卵母细胞中有一半能够形成原核样结构，因此在SCNT中通过适当的预处理可能促进古代动物组织核的细胞周期。这是首次报道的细胞核来源于猛犸组织的SCNT。
Here, we report the recovery of cell nuclei from 14,000–15,000 years old mammoth tissues and the injection of those nuclei into mouse enucleated matured oocytes by somatic cell nuclear transfer (SCNT). From both skin and muscle tissues, cell nucleus-like structures were successfully recovered. Those nuclei were then injected into enucleated oocytes and more than half of the oocytes were able to survive. Injected nuclei were not taken apart and remained its nuclear structure. Those oocytes did not show disappearance of nuclear membrane or premature chromosome condensation (PCC) at 1 hour after injection and did not form pronuclear-like structures at 7 hours after injection. As half of the oocytes injected with nuclei derived from frozen-thawed mouse bone marrow cells were able to form pronuclear-like structures, it might be possible to promote the cell cycle of nuclei from ancient animal tissues by suitable pre-treatment in SCNT. This is the first report of SCNT with nuclei derived from mammoth tissues.
影响因子：
3.6
作者：
Kuretake, S;Kimura, Y;Yanagimachi, R
通讯作者：
Yanagimachi, R