[11C]Acetate rest-stress protocol to assess myocardial perfusion and oxygen consumption reserve in a model of congestive heart failure in rats
[11C]Acetate rest-stress protocol to assess myocardial perfusion and oxygen consumption reserve in a model of congestive heart failure in rats
复制标题
DOI：
10.1016/j.nucmedbio.2011.07.010
复制
发表时间：
2012-02-01
影响因子：
3.1
通讯作者：
Benard, Francois
中科院分区：
文献类型：
--
作者：
Croteau, Etienne;Gascon, Suzanne;Benard, Francois
本研究描述了一种[C-11]乙酸盐静息-应激法，以获得间接估计心肌血流量（MBF）和心肌耗氧量（MVO 2）在大鼠。阿霉素心脏毒性被用来测试这种方法的有用性，用于评估充血性heart failure.Methods：[C-11]醋酸盐静息应激研究已被用于临床研究，以评估冠状动脉的能力，以应对压力。在这篇文章中，我们使用这种方法来评估阿霉素在大鼠模型中的心脏毒性。该方法首先在一组健康大鼠中验证，然后用于跟踪阿霉素化疗对心功能的影响。阿霉素对心肌灌注和氧耗储备的影响在休息和多巴酚丁胺stimulation.Results：验证的协议显示了良好的相关性之间的MBF和MVO 2（r（2）= 0.68）。多柔比星治疗组显示心血管灌注储备显著降低（P= 0.04），为1.3 +/- 0.2，而对照组为1.6 +/- 0.2。MVO 2储备也获得了类似的结果（治疗组1.8 +/- 0.4 vs对照组2.3 +/- 0.3; P= 0.02）。结论：我们描述了一种[C-11]醋酸盐PET静息应激方案，用于评估大鼠充血性心力衰竭及其应用于多柔比星化疗下心脏毒性的随访。这是一种快速可靠的方法来测量心脏灌注和耗氧量储备，可应用于开发新的策略，以减少蒽环类药物的心脏毒性。(C)2012 Elsevier Inc. All rights reserved.
This study describes an [C-11]acetate rest-stress method to obtain an indirect estimate of myocardial blood flow (MBF) and myocardial oxygen consumption (MVO2) in rats. Doxorubicin cardiotoxicity was used to test the usefulness of this approach for the assessment of congestive heart failure.Methods: [C-11]Acetate rest stress studies have been used in clinical research to assess the capacity of the coronary arteries to respond to stress. In this article, we used this approach to assess the cardiotoxicity of doxorubicin in a rat model. The method was first validated in a group of healthy rats and then used to follow the effect of doxorubicin chemotherapy on cardiac function. The effect of doxorubicin on myocardial perfusion and oxygen consumption reserve was measured at rest and under dobutamine stimulation.Results: Validation of the protocol showed a good correlation between the MBF and MVO2 (r(2)=.68). The doxorubicin-treated group showed a significant (P=.04) decrease in cardiovascular perfusion reserve at 1.3 +/- 0.2 compared with the control animals at 1.6 +/- 0.2. Similar results were obtained for the MVO2 reserve (treated 1.8 +/- 0.4 vs. controls 2.3 +/- 0.3; P=.02).Conclusions: We describe an [C-11]acetate PET rest stress protocol for the assessment of congestive heart failure in rats and its application to the follow-up of cardiotoxicity under doxorubicin chemotherapy. This is a rapid and reliable approach to the measurement of cardiac perfusion and oxygen consumption reserve that could be applied to the development of new strategies to reduce the cardiotoxicity of anthracycline. (C) 2012 Elsevier Inc. All rights reserved.