Very high-field EPR study of glycyl radical enzymes
Very high-field EPR study of glycyl radical enzymes
复制标题
DOI：
10.1021/ja026690j
复制
发表时间：
2003-01-08
影响因子：
15
通讯作者：
Heider, J
中科院分区：
文献类型：
--
作者：
Duboc-Toia, C;Hassan, AK;Heider, J
这项工作表明，非常高的领域EPR光谱允许一个相当准确的测定的g-张量的蛋白质自由基，包括C-中心的，因此可以用作探针区分酪氨酰，甘氨酰，或一个dahnyl-radical。在本文中，我们报告的第一个完整的分析的g-张量的甘氨酰自由基酶（厌氧核糖核苷酸还原酶，丙酮酸甲酸裂解酶，和苄基琥珀酸合成酶），从而提供了新的信息，其EPR性能。由于g-各向异性很小，只有在很高的场（18.8T）下才能得到g-张量的完全分辨。
This work shows that very high-field EPR spectroscopy allows a rather accurate determination of theg-tensor of protein radicals, including C-centered ones, and thus may be used as a probe for distinguishing a tyrosyl-, a glycyl-, or a tryptophanyl-radical. In this paper, we report the first complete analysis of theg-tensor of glycyl radical enzymes (anaerobic ribonucleotide reductase, pyruvate formate lyase, and benzylsuccinate synthase), thus providing new information on their EPR properties. Because theg-anisotropy is small, the complete resolution of theg-tensor could be only obtained at very high field (18.8 T).