A novel Exclusion mechanism for carbon resource partitioning in Aggregatibacter actinomycetemcomitans
A novel Exclusion mechanism for carbon resource partitioning in Aggregatibacter actinomycetemcomitans
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DOI：
10.1128/jb.00554-07
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发表时间：
2007-09-01
影响因子：
3.2
通讯作者：
Whiteley, Marvin
中科院分区：
文献类型：
--
作者：
Brown, Stacie A.;Whiteley, Marvin
伴放线聚集杆菌是人类口腔的常见共生菌，也是局限性侵袭性牙周炎的假定病原体。伴放线聚集杆菌是一种生长缓慢的细菌，其碳利用的代谢机制有限。这可能影响其在口腔定植的能力，因为口腔中的生长和群落组成是由碳的可利用性介导的。我们提供的证据表明，在体内相关的碳底物葡萄糖、果糖和乳酸存在的情况下，伴放线聚集杆菌优先代谢乳酸。尽管伴放线聚集杆菌在利用碳水化合物生长时生长更快且细胞产量更高，但它仍优先选择乳酸。对乳酸的偏好是由一种新的排斥机制介导的，即乳酸的代谢抑制碳水化合物的摄取。共培养研究表明，伴放线聚集杆菌利用口腔细菌戈登链球菌产生的乳酸，这表明口腔中可能存在交叉喂养现象。
The bacterium Aggregatibacter actinomycetemcomitans is a common commensal of the human oral cavity and the putative causative agent of the disease localized aggressive periodontitis. A. actinomycetemcomitans is a slow-growing bacterium that possesses limited metabolic machinery for carbon utilization. This likely impacts its ability to colonize the oral cavity, where growth and community composition is mediated by carbon availability. We present evidence that in the presence of the in vivo relevant carbon substrates glucose, fructose, and lactate A. actinomycetemcomitans preferentially metabolizes lactate. This preference for lactate exists despite the fact that A. actinomycetemcomitans grows faster and obtains higher cell yields during growth with carbohydrates. The preference for lactate is mediated by a novel exclusion mechanism in which metabolism of lactate inhibits carbohydrate uptake. Coculture studies reveal that A. actinomycetemcomitans utilizes lactate produced by the oral bacterium Streptococcus gordonii, suggesting the potential for cross-feeding in the oral cavity.