Ibotenate injections into the pre- and parasubiculum provide partial protection against kainate-induced epileptic damage in layer III of rat entorhinal cortex
Ibotenate injections into the pre- and parasubiculum provide partial protection against kainate-induced epileptic damage in layer III of rat entorhinal cortex
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DOI：
10.1046/j.1528-1157.2001.042007817.x
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发表时间：
2001-07-01
期刊：
影响因子：
5.6
通讯作者：
Schwarcz, R
中科院分区：
文献类型：
--
作者：
Eid, T;Du, F;Schwarcz, R
目的：内嗅皮层（EC）第三层神经元的丢失经常在颞叶癫痫患者和该疾病的动物模型中观察到。我们假设EC第III层对长时间癫痫活动的敏感性可能是由起源于前下托的兴奋性传入介导的。方法：实验设计通过局部注射鹅膏蕈氨酸来单侧消融前下托，并评估这种去传入对EC第III层神经元对化学惊厥剂红藻氨酸的脆弱性的影响结果：红藻氨酸治疗后，15只预先注射鹅膏蕈氨酸的大鼠中有11只在鹅膏蕈氨酸损伤同侧EC第III层显示出明确的部分神经保护作用。鹅膏蕈氨酸诱导的原发性病变的系列重建显示，内嗅神经元的保护，只有在动物中，有病变的前和旁的subiculum，特别是在深层（IV-VI）。结论：深层的前和旁的subiculum出现控制的神经元损伤EC层III。这种现象可能与癫痫的发生和颞叶癫痫的发病机制有关。
Purpose: A loss of neurons in layer III of the entorhinal cortex (EC) is often observed in patients with temporal lobe epilepsy and in animal models of the disorder. We hypothesized that the susceptibility of layer III of the EC to prolonged seizure activity might be mediated by excitatory afferents originating in the presubiculum.Methods: Experiments were designed to ablate the presubiculum unilaterally by focal ibotenate injections and to evaluate the effect of this deafferentation on the vulnerability of EC layer III neurons to the chemoconvulsant kainate (injected systemically 5 days later).Results: After treatment with kainate, 11 of the 15 rats preinjected with ibotenate showed clear-cut, partial neuroprotection in layer III of the EC ipsilateral to the ibotenate lesion. Serial reconstruction of the ibotenate-induced primary lesion revealed that entorhinal neurons were protected only in animals that had lesions in the pre- and parasubiculum, especially in the deep layers (IV-VI).Conclusions: The deep layers of the pre- and parasubiculum appear to control the seizure-induced damage of EC layer III. This phenomenon may be of relevance for epileptogenesis and for the pathogenesis of temporal lobe epilepsy.