Microporous collagen spheres produced via thermally induced phase separation for tissue regeneration
Microporous collagen spheres produced via thermally induced phase separation for tissue regeneration
复制标题
DOI：
10.1016/j.actbio.2009.08.044
复制
发表时间：
2010-03-01
影响因子：
9.7
通讯作者：
Day, Richard M.
中科院分区：
文献类型：
--
作者：
Keshaw, Hussila;Thapar, Nikhil;Day, Richard M.
胶原蛋白是在许多组织的细胞外基质中发现的丰富的蛋白质。由于其生物相容性，它是许多组织工程应用的潜在理想生物材料。然而，收获的胶原蛋白通常需要重建成三维基质，以促进诸如植入难以进入的组织腔中的应用。本研究的目的是生产一种适形的胶原基支架材料，能够支持组织再生，用于伤口修复应用。使用热诱导相分离（TIPS）技术制备微孔胶原球，并评估其生物相容性。胶原球成功地与戊二醛蒸汽交联，使其机械更稳定。当与肌成纤维细胞一起培养时，胶原球刺激血管生成生长因子、血管内皮生长因子（VEGF）的分泌的延长的显著增加，与单独的细胞相比，对照聚己内酯（PCL）球未能刺激VEGF分泌的类似延长的增加。使用鸡胚膜测定在体内也观察到增强的血管生成作用，其中与对照PCL球相比，观察到朝向胶原球会聚的血管数量显著增加。该研究的结果表明，使用TIPS生产的微孔胶原球具有生物活性，并且可以为难以接近的伤口中的组织再生提供新型适形支架（C）2009 Acta Materialia Inc.由Elsevier Ltd出版版权所有。
Collagen is an abundant protein found in the extracellular matrix of many tissues Due to its biocompatibility, it is a potentially ideal biomaterial for many tissue engineering applications However, harvested collagen often requires restructuring into a three-dimensional matrix to facilitate applications such as implantation into poorly accessible tissue cavities. The aim of the current study was to produce a conformable collagen-based scaffold material capable of supporting tissue regeneration for use in wound repair applications Microporous collagen spheres were prepared using a thermally induced phase separation (TIPS) technique and their biocompatibility was assessed The collagen spheres were successfully cross-linked with glutaraldehyde vapour, rendering them mechanically more stable. When cultured with myofibroblasts the collagen spheres stimulated a prolonged significant increase in secretion of the angiogenic growth factor, vascular endothelial growth factor (VEGF), compared with cells alone Control polycaprolactone (PCL) spheres failed to stimulate a similar prolonged increase in VEGF secretion. An enhanced angiogenic effect was also seen in vivo using the chick embryo membrane assay, where a significant increase in the number of blood vessels converging towards collagen spheres was observed compared with control PCL spheres. The results from this study indicate that microporous collagen spheres produced using TIPS are biologically active and could offer a novel conformable scaffold for tissue regeneration in poorly accessible wounds (C) 2009 Acta Materialia Inc. Published by Elsevier Ltd All rights reserved.