PLASMA CLEARANCE OF GLYCOPROTEINS WITH TERMINAL MANNOSE AND N-ACETYLGLUCOSAMINE BY LIVER NON-PARENCHYMAL CELLS - STUDIES WITH BETA-GLUCURONIDASE, N-ACETYL-BETA-D-GLUCOSAMINIDASE, RIBONUCLEASE-B AND AGALACTO-OROSOMUCOID
PLASMA CLEARANCE OF GLYCOPROTEINS WITH TERMINAL MANNOSE AND N-ACETYLGLUCOSAMINE BY LIVER NON-PARENCHYMAL CELLS - STUDIES WITH BETA-GLUCURONIDASE, N-ACETYL-BETA-D-GLUCOSAMINIDASE, RIBONUCLEASE-B AND AGALACTO-OROSOMUCOID
复制标题
DOI：
10.1042/bj1760103
复制
发表时间：
1978-01-01
影响因子：
4.1
通讯作者：
STAHL, P
中科院分区：
文献类型：
--
作者：
SCHLESINGER, PH;DOEBBER, TW;STAHL, P
末端非还原位置具有甘露糖和/或N-乙酰氨基葡萄糖的糖蛋白和各种溶酶体酶在静脉注射后被肝脏迅速从血浆中清除。行政。使用[大鼠]肝细胞分离技术来确定125I标记的β-葡萄糖醛酸酶、RNase B、无乳糖类粘蛋白和脱唾液酸类粘蛋白的细胞定位。在特定的放射性基础上，除 125I 标记的脱唾液酸类粘蛋白外，所有配体均富集在非实质细胞部分中。静脉注射后，固定分离的细胞并对其β-葡萄糖醛酸酶或N-乙酰基-β-D-氨基葡萄糖苷酶活性进行染色。注射酶后，显示出非实质细胞部分（可能是库普弗细胞）的富集。被非实质细胞摄取后，肝溶酶体β-葡萄糖醛酸酶和N-乙酰基-β-D-氨基葡萄糖苷酶的降解半衰期分别为2.2天和0.4天。
Glycoproteins having mannose and/or N-acetylglucosamine in the terminal non-reducing position and various lysosomal enzymes are rapidly cleared from plasma by the liver after i.v. administration. A [rat] liver cell-separation technique was used to determine the cellular localization of 125I-labeled .beta.-glucuronidase, RNase B, agalacto-orosomucoid and asialo-orosomucoid. On a specific radioactivity basis, all ligands except 125I-labeled asialo-orosomucoid were enriched in the non-parenchymal cell fraction. Isolated cells, fixed and stained for .beta.-glucuronidase or N-acetyl-.beta.-D-glucosaminidase activity after i.v. injection of the enzymes, showed enrichment in the non-parenchymal cell fraction (probably Kupffer cells). After uptake by the non-parenchymal cells, liver lysosomal .beta.-glucuronidase and N-acetyl-.beta.-D-glucosaminidase showed degradation half-times of 2.2 and 0.4 days respectively.