Landscape of meiotic crossovers in Hericium erinaceus
Landscape of meiotic crossovers in Hericium erinaceus
复制标题
猴头菇减数分裂交叉景观
DOI：
10.1016/j.micres.2020.126692
复制
发表时间：
2021-01-13
影响因子：
6.7
通讯作者：
Peng, Yuande
中科院分区：
文献类型：
--
作者：
Gong, Wenbing;Song, Xiaoya;Peng, Yuande
减数分裂交换显示出明显的种间和种内变异，而关于交换变异的分子机制的知识仍然有限。在此，我们描述了一个蘑菇形成真菌猴头菌的全基因组扫描的交叉。利用全基因组单核苷酸多态性（SNP）数据集的127个F-1单倍体后代，我们共定位了1316个交叉事件，发现它们更有可能发生在基因比基因间区域。超过30%的交换集中在59个交换热点中，这些交换热点优先位于靠近染色体末端的位置。然后，我们检查了交叉热点周围的基因组特征。结果表明，交叉热点与增加的基因密度和鸟嘌呤胞嘧啶（GC）含量。一个8 bp的GC丰富的基序（GCGTCAGC）被发现显着富集在这些热点。交配型位点的存在影响了局部尺度上的交叉，而不是总体交叉数。为了剖析形成交叉变异的遗传机制，我们对总交叉（TCO）和仅发生在热点（HCO）内的交叉事件进行了数量性状位点（QTL）定位。全基因组QTL定位结果表明，4个TCO-QTL和2个HCO-QTL均位于交叉热点内或附近。交叉变异由多个小效应位点形成，单个QTL贡献6.9%~ 11.7%的变异。一些重组途径基因，包括Spo 11，Msh 5，Smc 5被发现与定位的交叉QTL共定位。总之，这项研究的结果提供了深入了解的交叉分布和遗传因素赋予交叉变异的H。刺猬，并推进我们对蘑菇形成真菌减数分裂重组的认识。
Meiotic crossover shows marked interspecific and intraspecific variation, and knowledge about the molecular mechanism of crossover variation remains limited. Herein, we described the genome-wide scanning of crossover in one mushroom-forming fungus Hericium erinaceus. Utilizing the whole-genome single-nucleotide polymorphism (SNP) data-sets of a 127 F-1 haploid progeny, we localized a total of 1316 crossover events and found that they were more likely to occur in the genic than intergenic regions. More than 30 % of the crossovers were concentrated in 59 crossover hotspots that were preferentially located close to chromosome ends. We then examined the genomic features around crossover hotspots. Results showed that the crossover hotspots were associated with increased gene density and guanine-cytosine (GC) content. An 8-bp GC-rich motif (GCGTCAGC) was found to be significantly enriched in these hotspots. The presence of mating-type loci affected the crossover at local scale rather than the overall crossover number. In order to dissect the genetic mechanisms shaping crossover variation, we then conducted quantitative trait locus (QTL) mapping for the total crossovers (TCO) and the crossover events that solely occurred within hotspots (HCO). Genome-wide QTL scanning identified four TCO-QTLs and two HCO-QTLs, which all located within or next to the crossover-hotspots. Crossover variations were shaped by multiple small-effect loci, with individual QTL contributing 6.9 %-11.7 % of variation. A few recombination pathway genes, including Spo11, Msh5, and Smc5 were found to be co-localized with the mapped crossover QTLs. Taken together, findings of this study offer insights into the crossover distribution and genetic factors conferring crossover variation in H. erinaceus, and advance our understandings for meiotic recombination in mushroom-forming fungi.