Corneal endothelial function and structure following cryo-injury in the rabbit.
Corneal endothelial function and structure following cryo-injury in the rabbit.
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发表时间：
1984-12
影响因子：
4.4
通讯作者：
J. Minkowski;S. Bartels;F. Delori;S. Lee;K. Kenyon;A. H. Neufeld
中科院分区：
文献类型：
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作者：
J. Minkowski;S. Bartels;F. Delori;S. Lee;K. Kenyon;A. H. Neufeld
宽视野角膜内皮显微镜、荧光光度法、角膜厚度测定法和扫描电子显微镜用于表征兔角膜内皮损伤和恢复的可再现体内模型。在8mm中央冷冻损伤后，角膜保持增厚长达3周。平均内皮细胞对荧光素的渗透性在损伤后10天高于正常值，但在损伤后14天，内皮细胞对荧光素的渗透性与损伤前对照值无统计学显著差异，因此表明内皮细胞渗透性可能在损伤后约2周恢复正常。细胞形态，通过扫描电子显微镜测定，也基本上是正常的损伤后2周。内皮通透性似乎在间质厚度正常化之前恢复，表明内皮泵功能恢复滞后。
Wide-field specular microscopy, fluorophotometry, pachymetry, and scanning electron microscopy are used to characterize a reproducible, in vivo model of corneal endothelial injury and recovery in the rabbit. Following an 8-mm central cryo-injury, the cornea remains thickened for as long as 3 weeks. Mean endothelial permeability to fluorescein is above normal for 10 days following injury, but by 14 days postinjury the endothelial permeability to fluorescein is not statistically significantly different from preinjury control values, thus indicating that endothelial permeability probably returns to normal by approximately 2 weeks postinjury. Cell morphology, as determined by scanning electron microscopy, is also essentially normal by 2 weeks postinjury. Endothelial permeability appears to recover before stromal thickness normalizes, suggesting a lag in recovery of endothelial pump function.