Multiple Conserved Elements Structuring Inverted Repeats in the Mammalian Coat Color-Related Gene Asip
Multiple Conserved Elements Structuring Inverted Repeats in the Mammalian Coat Color-Related Gene Asip
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DOI：
10.2108/zs180081
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发表时间：
2019-02
影响因子：
0.9
通讯作者：
Yuki Sakuma;M. Matsunami;Toyoyuki Takada;Hitoshi Suzuki
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文献类型：
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作者：
Yuki Sakuma;M. Matsunami;Toyoyuki Takada;Hitoshi Suzuki
在家鼠（Mus musculus）的agglutinin信号传导基因蛋白（Asip）中，已知反向重复序列（IR）阵列存在于与腹侧特异性启动子区和伴随的两个外显子（外显子1A和1A′）相邻的非编码区中，这两个外显子位于外显子2、3和4的氨基酸编码区上游约100 kb处。为了确定哺乳动物Asip的基因结构并阐明其进化趋势，从数据库中检索并比较了6种啮齿动物（小鼠、大鼠、中国仓鼠、松鼠、豚鼠和裸鼹鼠）和3种非啮齿动物（兔、人和牛）的非编码序列。我们的同源性搜索分析显示存在三到五个高度保守的非编码元件（CNE）。这些CNE被发现在啮齿动物和兔形目动物中形成IR。IR的组合被进一步示出为构建对称的长IR阵列。三个通用CNE序列的种内和种间比较表明，物种内CNE对之间的同质性。这意味着某些进化限制维持了啮齿动物和兔子物种的IR结构。
In the agouti signaling gene protein (Asip) of the house mouse (Mus musculus), inverted repeat (IR) arrays are known to exist in a non-coding region adjacent to the ventral-specific promoter region and the accompanying two exons (exons 1A and 1A′), which are around 100 kb upstream from the amino acid coding regions of exons 2, 3, and 4. To determine the gene structure of mammalian Asip and to elucidate trends in its evolution, non-coding sequences of six rodent (mouse, rat, Chinese hamster, squirrel, guinea pig, and naked mole rat) and three non-rodent (rabbit, human, and cow) species were retrieved from databases and compared. Our homology search analyses revealed the presence of three to five highly conserved non-coding elements (CNE). These CNEs were found to form IRs in rodents and lagomorphs. Combinations of IRs were further shown to build symmetric, long IR arrays. Intra- and inter-specific comparisons of the sequences of three universal CNEs showed homogeneity between CNE pairs within species. This implies that certain evolutionary constraints maintained the IR structure in the rodent and rabbit species.