Differential protein profiles of Bradyrhizobium japonicum USDA110 bacteroid during soybean nodule development
Differential protein profiles of Bradyrhizobium japonicum USDA110 bacteroid during soybean nodule development
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DOI：
10.1111/j.1747-0765.2010.00500.x
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发表时间：
2010-08-01
影响因子：
2
通讯作者：
Tajima, Shigeyuki
中科院分区：
文献类型：
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作者：
Nomura, Mika;Arunothayanan, Hatthaya;Tajima, Shigeyuki
在豆科植物根瘤发育过程中，随着植物细胞形态的变化，根瘤菌发生显着的生化和生理变化并分化为类杆菌。入侵根瘤的拟杆菌不再是自由生长的细胞，而是依赖于植物细胞的所有资源。为阐明大豆慢生根瘤菌类杆菌分化的分子机制，采用双向凝胶电泳技术对大豆根瘤中类杆菌蛋白质进行了时程分析。利用蛋白质组学分析，蛋白质表达在大豆根瘤类杆菌接种后7，10，14，28，和49天（DAI）进行了监测。这些时间点与根瘤形成的早期阶段（7-10 DAI）、固氮的开始（14-28 DAI）和根瘤衰老（49 DAI）相一致。在这项研究中，275注释的蛋白质点被成功地确定，并进行聚类分析，其表达。在7或10 DAI时，在类杆菌中观察到大部分推定的上调蛋白质，并且这些上调蛋白质主要与转录、翻译、蛋白质折叠和降解有关。在类杆菌分化后期（14-28 DAI），Nif和Fix蛋白表达上调。此外，与伴侣蛋白和聚-β-羟基丁酸酯的合成酶相关的蛋白质以高丰度表达。一些蛋白质相关的溶质转运蛋白上调的类杆菌和占主导地位的检测整个结节的发展。这些蛋白质的相对丰度的变化进行了讨论，共生关系。
During nodule development of legumes, together with the morphological alteration of plant cells, the rhizobia undergo marked biochemical and physiological changes and differentiate to bacteroids. Bacteroids invading a nodule are no longer free-growing cells, instead depending on the plant cell for all resources. To elucidate the molecular mechanism of the bacteroid differentiation of Bradyrhizobium japonicum, a time-course analysis of bacteroid protein profiles was studied by 2-D gel electrophoresis in soybean nodules. Using proteomic analysis, protein expressions in soybean nodule bacteroids 7, 10, 14, 28, and 49 days after inoculation (DAI) were monitored. The time points coincide with the early stage of nodule formation (7-10 DAI), the onset of nitrogen fixation (14-28 DAI), and nodule senescence (49 DAI). In this study, 275 annotated protein spots were successfully identified, and a cluster analysis of their expression was performed. A large portion of putative, upregulated proteins were observed in bacteroids at 7 or 10 DAI, and these upregulated proteins were mainly related to transcription, translation, protein folding, and degradation. In the later period (14-28 DAI) of bacteroid differentiation, a number of Nif and Fix proteins were upregulated. In addition, proteins related to the chaperonin and a synthetic enzyme of the poly-beta-hydroxybutyrate were expressed at high abundance. A number of proteins related to solute transporter were upregulated in the bacteroids and dominantly detected throughout nodule development. Changes of relative abundance of these proteins are discussed in relation to symbiosis.