The additive effects of GS3 and qGL3 on rice grain length regulation revealed by genetic and transcriptome comparisons.
The additive effects of GS3 and qGL3 on rice grain length regulation revealed by genetic and transcriptome comparisons.
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通过遗传和转录组比较揭示 GS3 和 qGL3 对水稻粒长调节的累加效应
DOI：
10.1186/s12870-015-0515-4
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发表时间：
2015-06-24
影响因子：
5.3
通讯作者：
Zhang H
中科院分区：
文献类型：
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作者：
Gao X;Zhang X;Lan H;Huang J;Wang J;Zhang H
研究背景：粒长是决定水稻籽粒大小和粒形的重要性状，对水稻产量和外观品质有重要影响。结果：以93-11（gs 3/qGL 3）为遗传背景，通过常规回交和分子标记辅助选择（MAS），获得了两个近等基因系（NIL-GS 3（GS 3/qGL 3）和NIL-qgl 3（gs 3/qgl 3）），研究了两个主要粒长基因GS 3和qGL 3之间的遗传互作。利用NIL-GS 3与NIL-qgl 3杂交，利用MAS技术，获得了NIL-GS 3/qgl 3（GS 3/qgl 3）。通过比较93-11、NIL-GS 3、NIL-qgl 3和NIL-GS 3/qgl 3的粒长，基于双向方差分析，研究了GS 3、qGL 3和GS 3 × qGL 3互作对粒长的影响。结果表明，GS 3和qGL 3对水稻粒长的调控具有加性效应。对4个近等基因系的一级穗转录组进行比较分析，发现受GS 3和qGL 3影响的基因部分重叠，这两个基因座可能都参与油菜素内酯信号转导。结论：本研究为更好地理解水稻粒长调控机制提供了新的信息，有助于水稻育种者通过分子设计育种提高水稻产量和外观品质。
Background:Grain length, as a critical trait for rice grain size and shape, has a great effect on grain yield and appearance quality. Although several grain size/shape genes have been cloned, the genetic interaction among these genes and the molecular mechanisms of grain size/shape architecture have not yet to be explored.Results:To investigate the genetic interaction between two major grain length loci of rice, GS3 and qGL3, we developed two near-isogenic lines (NILs), NIL-GS3 (GS3/qGL3) and NIL-qgl3 (gs3/qgl3), in the genetic background of 93-11 (gs3/qGL3) by conventional backcrossing and marker-assisted selection (MAS). Another NIL-GS3/qgl3 (GS3/qgl3) was developed by crossing NIL-GS3 with NIL-qgl3 and using MAS. By comparing the grain lengths of 93-11, NIL-GS3, NIL-qgl3 and NIL-GS3/qgl3, we investigated the effects of GS3, qGL3 and GS3 × qGL3 interaction on grain length based on two-way ANOVA. We found that GS3 and qGL3 had additive effects on rice grain length regulation. Comparative analysis of primary panicle transcriptomes in the four NILs revealed that the genes affected by GS3 and qGL3 partially overlapped, and both loci might be involved in brassinosteroid signaling.Conclusion:Our data provide new information to better understand the rice grain length regulation mechanism and help rice breeders improve rice yield and appearance quality by molecular design breeding.