Photoinduced affinity labeling of the Escherichia coli ribosome puromycin site.
Photoinduced affinity labeling of the Escherichia coli ribosome puromycin site.
复制标题
大肠杆菌核糖体嘌呤霉素位点的光诱导亲和标记。
作者：
E. N. Jaynes;P. G. Grant;G. Giangrande;R. Wieder;B. Cooperman
The photoincorporation of puromycin into Escherichia coli ribosomes has been studied in detail. Incorporation into protein L23 as a function of puromycin concentration follows a simple saturation curve and is specifically blocked by structural and functional analogues of puromycin, thus demonstrating that such incorporation proceeds via an affinity labeling process. Incorporation into L23 becomes more specific as the light fluence is reduced, indicating that such incorporation takes place from a native rather than light-denatured puromycin site. L23 remains the major labeled protein using ribosomes prepared by several procedures, suggesting the conservative nature of the site. In addition evidence is presented for affinity labeling of S14 and of a site in the RNA fraction of the 50S particle. Specific incorporation appears to proceed with an anomalously high quantum yield. The detailed photochemical mechanism is not understood, although 8-alkylation of purine moiety has been excluded. Incorporation is largely inhibited in the presence of thiol reagents.
