Some high-performance liquid-chromatographic studies of the metabolism of aflatoxins by rat liver microsomal preparations.
Some high-performance liquid-chromatographic studies of the metabolism of aflatoxins by rat liver microsomal preparations.
复制标题
大鼠肝微粒体制剂代谢黄曲霉毒素的一些高效液相色谱研究。
DOI：
10.1042/bj1740839
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发表时间：
1978
期刊：
影响因子：
--
通讯作者：
P. J. Colley
中科院分区：
文献类型：
--
作者：
G. Neal;P. J. Colley
在大鼠肝微粒体制剂中检测了黄曲霉毒素 B1 的体外代谢。 2. H.p.l.c.使用（高效液相色谱）系统。使用硅胶柱分离非极性代谢物。还开发了一种利用反相柱分离极性和非极性代谢物的系统。 3. 黄曲霉毒素B1的主要代谢物为黄曲霉毒素M1、黄曲霉毒素Q1以及与黄曲霉毒素B1降解产物2,3-二氢二醇进行共色谱分析的化合物。 4.检查了从对照和苯巴比妥预处理的大鼠中分离的微粒体制剂代谢黄曲霉毒素B1的时间过程。后者的微粒体制剂的代谢速率和程度更大。苯巴比妥预处理使黄曲霉毒素Q1的形成增强了4--5倍，而黄曲霉毒素M1的产生仅增加了1--2倍。通过苯巴比妥预处理，黄曲霉毒素B1 2,3-二氢二醇的降解产物的形成增加了4--5倍。 5.检测黄曲霉毒素M1、P1、Q1的微粒体代谢。黄曲霉毒素 M1 显然经历了非常有限的微粒体代谢，转变成极性更强的化合物。 Aflatoxin P1 was not metabolized.黄曲霉毒素 Q1 的情况很复杂，因为它在缺乏 NADPH 的情况下被代谢成一种未鉴定的代谢物。仅当NADPH存在时，黄曲霉毒素B1才作为黄曲霉毒素Q1的代谢物出现，并且随后还观察到更多极性代谢物的形成。
The metabolism of aflatoxin B1 in vitro was examined in rat liver microsomal preparations. 2. H.p.l.c. (high-performance liquid-chromatographic) systems were used. A silica column was used to separate non-polar metabolites. A system utilizing a reversed-phase column which separates both poar and non-polar metabolites was also developed. 3. The principal metabolites of aflatoxin B1 found were aflatoxin M1, aflatoxin Q1 and a compound which co-chromatographed with a degradation product of aflatoxin B1 2,3-dihydrodiol. 4. The time course of metabolism of aflatoxin B1 by microsomal preparations isolated from control and phenobarbitone-pretreated rats was examined. The rate and extent of metabolism was greater with microsomal preparations from the latter. The formation of aflatoxin Q1 was enhanced 4--5-fold by phenobarbitone pretreatment, whereas the production of aflatoxin M1 was only increased 1--2-fold. The formation of the degradation product of aflatoxin B1 2,3-dihydrodiol was increased 4--5-fold by the pretreatment with phenobarbitone. 5. The microsomal metabolism of aflatoxins M1, P1 and Q1 was examined. Aflatoxin M1 apparently underwent very limited microsomal metabolism to more polar compounds. Aflatoxin P1 was not metabolized. The situation with aflatoxin Q1 was complicated in that it was metabolized in the absence of NADPH to an unidentified metabolite. Aflatoxin B1 appeared as a metabolite of aflatoxin Q1 only when NADPH was present, and the formation of more polar metabolites was also then observed.