Using protein-binding microarrays to study transcription factor specificity: homologs, isoforms and complexes
Using protein-binding microarrays to study transcription factor specificity: homologs, isoforms and complexes
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DOI：
10.1093/bfgp/elu046
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发表时间：
2015-01-01
影响因子：
4
通讯作者：
Siggers, Trevor
中科院分区：
文献类型：
--
作者：
Andrilenas, Kellen K.;Penvose, Ashley;Siggers, Trevor
蛋白质DNA结合是基因调控特异性的核心，表征转录因子（IF） DNA结合的方法对调控特异性的研究至关重要。高通量（HT）技术通过显着增加可以进行的结合测量的数量，彻底改变了我们表征蛋白质DNA结合的能力。蛋白质结合微阵列（PBMs）是研究tf dna结合特异性的一个强大的HT平台。对pbm确定的dna结合谱的分析为TF结合多样性的范围和机制提供了新的见解。本文将重点介绍PBM技术，并讨论其在TF特异性研究中的应用，特别是TF同源物和多蛋白复合物的结合多样性。
Protein DNA binding is central to specificity in gene regulation, and methods for characterizing transcription factor (IF) DNA binding remain crucial to studies of regulatory specificity. High-throughput (HT) technologies have revolutionized our ability to characterize protein DNA binding by significantly increasing the number of binding measurements that can be performed. Protein-binding microarrays (PBMs) are a robust and powerful HT platform for studying DNA-binding specificity of TFs. Analysis of PBM-determined DNA-binding profiles has provided new insight into the scope and mechanisms of TF binding diversity. In this review, we focus specifically on the PBM technique and discuss its application to the study of TF specificity, in particular, the binding diversity of TF homologs and multi-protein complexes.