Utility of a paraffin section-reactive CD56 antibody (123C3) for characterization and diagnosis of lymphomas.
Utility of a paraffin section-reactive CD56 antibody (123C3) for characterization and diagnosis of lymphomas.
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使用石蜡切片反应性 CD56 抗体 (123C3) 来表征和诊断淋巴瘤。
DOI：
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发表时间：
1996
影响因子：
5.6
通讯作者：
M. Pau
中科院分区：
文献类型：
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作者：
W. Tsang;J. Chan;C. Ng;M. Pau
虽然CD 56（神经细胞粘附分子，自然杀伤细胞标志物）的表达在淋巴瘤中并不常见，但这一特征定义了一种独特而重要的淋巴瘤类别：假定的自然杀伤（NK）细胞淋巴瘤，其显示出上呼吸消化道，皮肤，骨骼肌和其他淋巴结部位的偏好，并追求积极的临床过程。到目前为止，CD 56表达只能在新鲜或冷冻组织上进行可靠分析。在这项研究中，我们评估的灵敏度和特异性的CD 56抗体，123 C3，当应用于常规福尔马林固定，石蜡包埋组织的淋巴瘤分析，通过比较染色结果与冷冻组织上获得的使用CD 56抗体NKH 1。123 C3抗体仅在使用高压锅或微波炉进行抗原修复的情况下才对石蜡切片起作用。32例CD 56 + T/NK细胞淋巴瘤和1例CD 56 + B淋巴母细胞淋巴瘤的瘤细胞123 C3均呈脆膜染色。24例CD 56-T细胞淋巴瘤和50例CD 56-B细胞淋巴瘤均未被123 C3染色。在不同部位的正常或反应性淋巴组织中，很少有小淋巴细胞（< 0.1%）显示123 C3细胞膜染色，尽管偶尔浆细胞可能显示胞质染色。我们的结论是，适当的抗原修复程序，123 C3可以可靠地应用于常规石蜡切片检测淋巴瘤中的CD 56表达。此外，该抗体可用于支持淋巴瘤的诊断或检测CD 56 + T/NK细胞淋巴瘤病例的残留疾病，其中肿瘤性淋巴样细胞较小且显示最小的淋巴结，特别是在小活检中。
Although expression of CD56 (neural cell adhesion molecule, a natural killer cell marker) is uncommon among lymphomas, this feature has defined a distinctive and important category of lymphoma: the putative natural killer (NK) cell lymphoma, which shows a predilection for the upper aerodigestive tract, skin, skeletal muscle, and other extranodal sites and pursues an aggressive clinical course. Thus far, CD56 expression can be reliably analyzed only on fresh or frozen tissues. In this study, we evaluated the sensitivity and specificity of a CD56 antibody, 123C3, when applied on routine formalin-fixed, paraffin-embedded tissues for analysis of lymphomas, by comparing the staining results with those obtained on frozen tissues using the CD56 antibody NKH1. The 123C3 antibody worked on paraffin sections only with prior antigen retrieval using a pressure cooker or a microwave oven. Among 32 CD56+ T/NK cell lymphomas and one CD56+ B-lymphoblastic lymphoma, the neoplastic cells showed crisp membrane staining with 123C3 in all cases. None of the 24 CD56- T-cell lymphomas and 50 CD56-B-cell lymphomas stained with 123C3. In normal or reactive lymphoid tissues from a variety of sites, there were few small lymphocytes (< 0.1%) that showed cell membrane staining with 123C3, although occasional plasma cells might show cytoplasmic staining. We conclude that with suitable antigen retrieval procedures, 123C3 can be reliably applied on routine paraffin sections for detection of CD56 expression in lymphomas. Furthermore, this antibody can be used to support a diagnosis of lymphoma or to detect residual disease for cases of CD56+ T/NK cell lymphoma in which the neoplastic lymphoid cells are small and show minimal atypia, especially in small biopsies.
影响因子：
4.4
作者：
L. Lanier;J. Phillips;J. Hackett;M. Tutt;V. Kumar
通讯作者：
L. Lanier;J. Phillips;J. Hackett;M. Tutt;V. Kumar
CD3、CD56 大颗粒淋巴细胞白血病的侵袭性变体。
影响因子：
20.3
作者：
Gentile,TC;Uner,AH;Hutchison,RE;Wright,J;Ben-Ezra,J;Russell,EC;LoughranJr,TP
通讯作者：
LoughranJr,TP