Polymerization of the Actin-Like Protein MamK, Which Is Associated with Magnetosomes
Polymerization of the Actin-Like Protein MamK, Which Is Associated with Magnetosomes
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DOI：
10.1128/jb.00899-07
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发表时间：
2007-09
影响因子：
3.2
通讯作者：
A. Taoka;Ryuji Asada;Long-Fei Wu;Y. Fukumori
中科院分区：
文献类型：
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作者：
A. Taoka;Ryuji Asada;Long-Fei Wu;Y. Fukumori
摘要从大肠杆菌中纯化了重组肌动蛋白样蛋白MamK，并以其为抗原制备了抗MamK抗体。免疫组织化学染色显示MamK在磁螺杆菌细胞中呈线性分布，并与磁小体相关。此外，我们还证明了MamK在体外可以聚合成丝状束。
ABSTRACT The recombinant actin-like protein MamK was purified from Escherichia coli and used as an antigen to generate the anti-MamK antibody. Immunostaining studies showed a linear distribution of MamK in Magnetospirillum magnetotacticum cells and of MamK in association with magnetosomes. Moreover, we demonstrated that MamK polymerizes into filamentous bundles in vitro.