[Study on role of TIGIT signal in Th1/Th2 balance in Schistosoma japonicum-infected mice].
[Study on role of TIGIT signal in Th1/Th2 balance in Schistosoma japonicum-infected mice].
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DOI：
10.16250/j.32.1374.2017233
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发表时间：
2018-04-20
期刊：
影响因子：
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通讯作者：
Chuan, Su
中科院分区：
文献类型：
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作者：
Li-Na, Zhang;Xiao-Fan, Wang;Chuan, Su
目的：探讨日本血吸虫感染过程中TIGIT信号在Th1/Th2平衡中的作用。方法：用日本血吸虫尾蚴感染雄性C57BL/6小鼠，并以正常未感染小鼠作为对照。评估小鼠脾脏中 TIGIT+ 细胞、Ki67+CD3+CD4+TIGIT+ 细胞、Ki67+CD3+CD4+TIGIT- 细胞、IFN-gamma+CD3+CD4+TIGIT+ 细胞、IFN-gamma+CD3+CD4+TIGIT- 细胞、IL-4+CD3+CD4+TIGIT+ 细胞和 IL-4+CD3+CD4+TIGIT- 细胞的百分比结果：日本血吸虫感染小鼠脾脏中TIGIT+CD4+ T细胞比例高于正常未感染小鼠（29.30%±0.70% vs. 3.09%±0.50%；t=8.834，P < 0.01）。但感染组与正常对照组之间TIGIT+ CD8+ T细胞比例无显着差异（3.61%±0.26% vs. 3.58%±0.16%；t=0.108，P > 0.05），感染组与对照组之间TIGIT+细胞在非T细胞中的比例也无显着差异（1.86%±0.19%）。与1.37%±0.17%；t=1.931，P>0.05）。此外，TIGIT+细胞中Ki67的比例高于TIGIT-细胞（17.03%±0.64%比6.59%±0.37%；t=14.09，P<0.01）。 TIGIT+CD4+ T 细胞中 Th2/Th1 比值高于 TIGIT-CD4+ T 细胞（39.28%±3.75% vs. 11.79%±1.64%；t = 6.721，P < 0.01）。结论：TIGIT 信号可能参与了日本血吸虫感染小鼠 Th2 反应的发生。
OBJECTIVE: To investigate the role of TIGIT signal in Th1/Th2 balance in the process of Schistosoma japonicum infection.METHODS: Male C57BL/6 mice were infected with cercariae of S. japonicum, and normal uninfected mice served as the controls. The percentages of TIGIT+ cells, Ki67+CD3+CD4+TIGIT+ cells, Ki67+CD3+CD4+TIGIT- cells, IFN-gamma+CD3+CD4+TIGIT+ cells, IFN-gamma+CD3+CD4+TIGIT- cells, IL-4+CD3+CD4+TIGIT+ cells and IL-4+CD3+CD4+TIGIT- cells were evaluated in mouse spleen by flow cytometry.RESULTS: The proportion of TIGIT+CD4+ T cells was higher in the spleen of S. japonicum-infected mice than in the normal uninfected mice (29.30%±0.70% vs. 3.09%±0.50%; t = 8.834, P < 0.01) . However, no significant difference in the percentages of TIGIT+ CD8+ T cells between the infection group and normal controls (3.61%±0.26% vs. 3.58%±0.16%; t = 0.108, P > 0.05), and no significant difference was detected in the percentages of TIGIT+ cells in non-T cells between the infection group and controls (1.86%±0.19% vs.1.37%±0.17%; t = 1.931, P > 0.05) . In addition, the proportion of Ki67 in the TIGIT+ cells was higher than that in the TIGIT- cells (17.03%±0.64% vs. 6.59%±0.37%; t = 14.09, P < 0.01) . The Th2/Th1 ratio was higher in the TIGIT+CD4+ T cells than in the TIGIT-CD4+ T cells (39.28%±3.75% vs. 11.79%±1.64%; t = 6.721, P < 0.01) .CONCLUSIONS: The TIGIT signaling may be involved in the development of Th2 responses in the mice infected with S. japonicum.