WISP-2 expression in human salivary gland tumors.
WISP-2 expression in human salivary gland tumors.
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DOI：
10.3892/ijmm.17.4.567
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发表时间：
2006-04
影响因子：
5.4
通讯作者：
Y. Kouzu;K. Uzawa;Masaki Kato;M. Higo;Y. Nimura;K. Harada;T. Numata;N. Seki;Mitsunobu Sato;H. Tanzawa
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文献类型：
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作者：
Y. Kouzu;K. Uzawa;Masaki Kato;M. Higo;Y. Nimura;K. Harada;T. Numata;N. Seki;Mitsunobu Sato;H. Tanzawa
本研究旨在揭示唾液腺肿瘤（sgt）的详细遗传机制，以开发新的独立标志物。我们构建了一个内部cDNA微阵列，携带来自SGT和口腔鳞状细胞癌cDNA文库的2201个cDNA克隆。使用该微阵列系统分析了来自sgt衍生细胞系的四个细胞系。我们的微阵列系统在其他类型的人类癌细胞系和临床样本（10例正常唾液腺，11例多形性腺瘤，10例腺样囊性癌和3例腺癌）中进一步分析了鉴定的基因的mRNA或蛋白表达水平。与对照RNA相比，共鉴定出2个上调基因和6个下调基因。在上调的基因中，我们选择了在乳腺癌发生中起重要作用的WISP-2进行进一步分析。我们发现，与其他类型的人类癌细胞系相比，sgt衍生细胞系中WISP-2基因的表达更高。此外，WISP-2 mRNA和蛋白在nsg中的表达水平显著高于sgt。这些结果表明，WISP-2可能是一个可靠的独立标志物，WISP-2基因的下调或缺失可能与sgt的发生有关。
This study was designed to disclose detailed genetic mechanisms in salivary gland tumors (SGTs) for development of novel independent marker. We constructed an in-house cDNA microarray carrying 2,201 cDNA clones derived from SGT and oral squamous cell carcinoma cDNA libraries. Four cell lines that originated from the SGT-derived cell lines were analyzed using this microarray system. The genes identified by our microarray system were further analyzed at the mRNA or protein expression level in other types of human cancer cell lines and clinical samples (ten normal salivary glands [NSGs], eleven pleomorphic adenomas, ten adenoid cystic carcinomas and three adenocarcinomas). Two up-regulated genes and six down-regulated genes were identified in common when compared with the control RNA. Of the up-regulated genes, WISP-2, which plays an important role in breast carcinogenesis, was selected for further analyses. We found a higher expression of the WISP-2 gene in the SGT-derived cell lines compared with other types of human cancer cell lines. Furthermore, WISP-2 mRNA and protein expression levels in NSGs were significantly higher than those in SGTs. These results suggest that WISP-2 could be a reliable independent marker and that down-regulation or loss of the WISP-2 gene may be associated with the development of SGTs.