Cloning and characterization of E2F-2, a novel protein with the biochemical properties of transcription factor E2F
Cloning and characterization of E2F-2, a novel protein with the biochemical properties of transcription factor E2F
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DOI：
10.1128/mcb.13.12.7802-7812.1993
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发表时间：
1993-12
影响因子：
5.3
通讯作者：
M. Ivey-Hoyle;R. Conroy;H. Huber;P. Goodhart;A. Oliff;D. Heimbrook
中科院分区：
文献类型：
--
作者：
M. Ivey-Hoyle;R. Conroy;H. Huber;P. Goodhart;A. Oliff;D. Heimbrook
E2 F是一种哺乳动物转录因子，在细胞周期调控中起重要作用。虽然已经克隆了至少两种具有E2 F样活性的蛋白质（E2 F-1和DP-1），但几个实验室的研究表明可能存在其他同源物。以E2 F-1（K. Helin，J. A. Lees，M. Vidal，N. Dyson，E. Harlow和A. Fattaey，Cell 70：337-350，1992）。E2 F-2与E2 F-1具有总体46%的氨基酸同一性。E2 F-1的DNA和视网膜母细胞瘤基因产物结合结构域的序列和功能在E2 F-2中是保守的。E2 F-2的DNA结合活性通过与特定的十二烷基硫酸钠-聚丙烯酰胺凝胶电泳纯化的HeLa细胞E2 F组分互补而显著增强，并且抗E2 F-2抗体与纯化的HeLa细胞E2 F组分交叉反应。这些观察结果与E2 F作为两种不同蛋白质的异二聚体结合DNA的模型一致，并且E2 F-2在功能和免疫学上与这些蛋白质之一相关。
E2F is a mammalian transcription factor that appears to play an important role in cell cycle regulation. While at least two proteins (E2F-1 and DP-1) with E2F-like activity have been cloned, studies from several laboratories suggest that additional homologs may exist. A novel protein with E2F-like properties, designated E2F-2, was cloned by screening a HeLa cDNA library with a DNA probe derived from the DNA binding domain of E2F-1 (K. Helin, J. A. Lees, M. Vidal, N. Dyson, E. Harlow, and A. Fattaey, Cell 70:337-350, 1992). E2F-2 exhibits overall 46% amino acid identity to E2F-1. Both the sequence and the function of the DNA and retinoblastoma gene product binding domains of E2F-1 are conserved in E2F-2. The DNA binding activity of E2F-2 is dramatically enhanced by complementation with particular sodium dodecyl sulfate-polyacrylamide gel electrophoresis-purified components of HeLa cell E2F, and anti-E2F-2 antibodies cross-react with components of purified HeLa cell E2F. These observations are consistent with a model in which E2F binds DNA as a heterodimer of two distinct proteins, and E2F-2 is functionally and immunologically related to one of these proteins.