Effects of different types of exercise on skeletal muscle atrophy, antioxidant capacity and growth factors expression following myocardial infarction
Effects of different types of exercise on skeletal muscle atrophy, antioxidant capacity and growth factors expression following myocardial infarction
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不同运动类型对心肌梗死后骨骼肌萎缩、抗氧化能力及生长因子表达的影响
DOI：
10.1016/j.lfs.2018.10.015
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发表时间：
2018-11-15
期刊：
影响因子：
6.1
通讯作者：
Tian, Zhenjun
中科院分区：
文献类型：
--
作者：
Cai, Mengxin;Wang, Qing'an;Tian, Zhenjun
目的：心肌梗死（MI）伴有骨骼肌异常。目的是探索一种改善心功能和预防骨骼肌萎缩的最佳运动模式，并检测运动诱导抑制肌肉萎缩的可能机制。主要方法：大鼠在心肌梗死术后进行为期四周的不同类型运动（阻力训练，RT；中强度连续有氧运动，MCE和高强度间歇有氧运动，HIA）。检测骨骼肌的心功能、心脏和骨骼肌的组织学变化、氧化应激、抗氧化能力和肌肉萎缩相关因子的表达。主要发现：三种类型的运动不同程度地改善了心脏功能，减少了心脏纤维化，增加了肌肉重量和肌纤维的横截面面积（CSA）。RT和MCE干预心肌梗死大鼠的存活率均高于HIA。运动可下调murf1和atrogin-1 mRNA水平，降低活性氧水平，提高抗氧化能力，调节胰岛素样生长因子1 （IGF1）、机械生长因子（MGF）、神经调节因子1 （NRG1）和肌生长抑制素（MSTN）的表达，激活比目鱼肌Akt和Erk1/2信号。肌纤维CSA及骨骼肌中IGF1、MGF、NRG1的表达与心功能有相关性。意义：RT和MCE是心肌梗死后早期运动康复的首选方案，各类运动均可通过提高骨骼肌抗氧化能力、减少氧化应激和蛋白降解、调节骨骼肌生长因子表达等方式有效抑制骨骼肌萎缩。
Aims: Myocardial infarction (MI) is accompanied with skeletal muscle abnormalities. The aims are to explore an optimal exercise mode to improve cardiac function and prevent skeletal muscle atrophy, and detect the possible mechanisms of exercise-induced inhibition of muscle atrophy.Main methods: Rats were subjected to four weeks of different types of exercise after MI surgery (resistance training, RT; moderated-intensity continuous aerobic exercise, MCE and high-intensity intermittent aerobic exercise, HIA). Cardiac function, histological changes of heart and skeletal muscle, oxidative stress, antioxidant capacity and the expression of muscle atrophy-related factors were detected in skeletal muscle.Key findings: The three types of exercise improved heart function, reduced cardiac fibrosis and increased muscle weight and cross-section area (CSA) of muscle fibers in different degrees. The survival rates of MI rats intervened by RT and MCE were higher than HIA. Exercise down-regulated the mRNA levels of murf1 and atrogin-1, decreased reactive oxygen species level, increased antioxidant capacity, regulated the expression of insulin-like growth factor 1 (IGF1), mechano growth factor (MGF), Neuregulin1 (NRG1) and Myostatin (MSTN), and activated Akt and Erk1/2 signalings in soleus muscle. Furthermore, CSA of muscle fibers and the expression of IGF1, MGF, NRG1 in skeletal muscle had correlations with cardiac function.Significance: RT and MCE are the first two choices for the early exercise rehabilitation following MI. All types of exercise can effectively inhibit skeletal muscle atrophy through increasing the antioxidant capacity, reducing oxidative stress and protein degradation, and regulating the growth factors expression in skeletal muscle.