The eps15 homology (EH) domain-based interaction between eps15 and hrb connects the molecular machinery of endocytosis to that of nucleocytosolic transport.
The eps15 homology (EH) domain-based interaction between eps15 and hrb connects the molecular machinery of endocytosis to that of nucleocytosolic transport.
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EPS15与EPS15和HRB之间的EPS15同源性（EH）结构域的相互作用将内吞作用的分子机制连接到核细胞胞浆转运的分子机制。
DOI：
10.1083/jcb.147.7.1379
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发表时间：
1999-12-27
影响因子：
7.8
通讯作者：
Di Fiore, P P
中科院分区：
文献类型：
--
作者：
Doria, M;Salcini, A E;Colombo, E;Parslow, T G;Pelicci, P G;Di Fiore, P P
Eps 15同源性（EH）模块是蛋白质-蛋白质相互作用结构域，其建立参与内吞作用和分选的各个方面的连接网络。EH蛋白与Hrb（Rev蛋白的细胞辅因子）和相关蛋白Hrbl结合的发现提出了EH网络也可能影响所谓的Rev输出途径的可能性，该途径介导蛋白质和RNA的核质转移。在这项研究中，我们证明，Eps 15和Eps 15 R，两个含EH的蛋白质，协同Hrb和Hrbl，以增强功能的Rev的出口途径。此外，EH介导的关联Eps 15和Hrb之间的协同效应是必需的。Eps 15和Hrb之间的相互作用发生在细胞质中，因此指出了Hrb的一个意想不到的作用位点，以及Eps 15-Hrb复合物在调节Rev稳定性中的可能作用。
The Eps15 homology (EH) module is a protein–protein interaction domain that establishes a network of connections involved in various aspects of endocytosis and sorting. The finding that EH-containing proteins bind to Hrb (a cellular cofactor of the Rev protein) and to the related protein Hrbl raised the possibility that the EH network might also influence the so-called Rev export pathway, which mediates nucleocytoplasmic transfer of proteins and RNAs. In this study, we demonstrate that Eps15 and Eps15R, two EH-containing proteins, synergize with Hrb and Hrbl to enhance the function of Rev in the export pathway. In addition, the EH-mediated association between Eps15 and Hrb is required for the synergistic effect. The interaction between Eps15 and Hrb occurs in the cytoplasm, thus pointing to an unexpected site of action of Hrb, and to a possible role of the Eps15–Hrb complex in regulating the stability of Rev.