Activation of murine epidermal gamma delta T cells through surface 2B4.
Activation of murine epidermal gamma delta T cells through surface 2B4.
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通过表面 2B4 激活小鼠表皮 γ δ T 细胞。
DOI：
10.1002/eji.1830250440
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发表时间：
1995
期刊：
影响因子：
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通讯作者：
Takashima,A
中科院分区：
文献类型：
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作者：
Schuhmachers,G;Ariizumi,K;Mathew,PA;Bennett,M;Kumar,V;Takashima,A
树突状表皮 T 细胞 (DETC) 是通常存在于小鼠皮肤中的 γδ T 细胞。它们在其表面表达 2B4 分子，这是一种免疫球蛋白基因超家族的 66 kDa 糖蛋白，被认为与自然杀伤细胞和淋巴因子激活杀伤细胞的抗肿瘤细胞毒性有关。在这里，我们证明表面 2B4 的连接可以转导 DETC 中的细胞激活信号。用抗2B4单克隆抗体治疗会触发DETC细胞系分泌干扰素-γ和白细胞介素-2，诱导静息DETC细胞系增殖，放大从小鼠皮肤中新鲜分离的DETC的抗CD3依赖性增殖；并上调egr-1和c-fos mRNA表达。这些结果表明 DETC 激活的独特途径。
Dendritic epidermal T cells (DETC) are γδ T cells that normally reside in murine skin. They express on their surface the 2B4 molecule, a 66‐kDa glycoprotein of the immunoglobulin gene superfamily thought to be associated with anti‐tumor cytotoxicity by natural killer and lymphokine‐activated killer cells. Here, we show that ligation of surface 2B4 transduces cell activation signals in DETC. Treatment with anti‐2B4 monoclonal antibodies triggers the secretion of interferon‐γ and interleukin‐2 by DETC lines, induces proliferation of resting DETC lines, amplifies anti‐CD3‐dependent proliferation of DETC freshly isolated from mouse skin; and up‐regulates egr‐1 and c‐fos mRNA expression. These results indicate a unique pathway for DETC activation.