Interlinking showy traits: co-engineering of scent and colour biosynthesis in flowers
Interlinking showy traits: co-engineering of scent and colour biosynthesis in flowers
复制标题
DOI：
10.1111/j.1467-7652.2008.00329.x
复制
发表时间：
2008-05-01
影响因子：
13.8
通讯作者：
Vainstein, Alexander
中科院分区：
文献类型：
--
作者：
Ben Zvi, Michal Moyal;Florence, Negre-Zakharov;Vainstein, Alexander
苯丙素途径产生决定花色和香味的代谢物。这些代谢物是植物吸引传粉者的主要手段之一，从而保证植物的生存。由于缺乏对调节气味产生的因素的了解，阻碍了花卉中挥发性苯丙素产量的成功提高。在这项研究中，来自拟南芥的花青素色素 1 (Pap1) Myb 转录因子能够调节非挥发性苯丙素（包括花青素）的产生，并被稳定引入矮牵牛中。除了色素沉着增加之外，Pap1 转基因矮牵牛花的挥发性苯丙素/苯类化合物的产量增加了十倍。挥发物产量的急剧增加与矮牵牛挥发物产量的本地夜间节律相对应。将苯丙氨酸应用于 Pap1 转基因花后，白天释放的挥发物水平可忽略不计，增加到夜间水平。基于基因表达谱和途径中间体的水平，有人提出，气味和颜色基因的代谢通量增加和转录激活是Pap1增强矮牵牛花颜色和气味产生的基础。参与重要植物功能的途径内或途径之间代谢步骤的协调调节，如本文所示，决定植物与传粉者相互作用的两个显着性状，为植物生存提供了明显的优势。使用激活气味产生的调节因子为香味代谢结构创造了一种新的生物技术策略，从而为育种目的创造了新的遗传变异。
The phenylpropanoid pathway gives rise to metabolites that determine floral colour and fragrance. These metabolites are one of the main means used by plants to attract pollinators, thereby ensuring plant survival. A lack of knowledge about factors regulating scent production has prevented the successful enhancement of volatile phenylpropanoid production in flowers. In this study, the Production of Anthocyanin Pigment1 (Pap1) Myb transcription factor from Arabidopsis thaliana, known to regulate the production of non-volatile phenylpropanoids, including anthocyanins, was stably introduced into Petunia hybrida. In addition to an increase in pigmentation, Pap1-transgenic petunia flowers demonstrated an increase of up to tenfold in the production of volatile phenylpropanoid/benzenoid compounds. The dramatic increase in volatile production corresponded to the native nocturnal rhythms of volatile production in petunia. The application of phenylalanine to Pap1-transgenic flowers led to an increase in the otherwise negligible levels of volatiles emitted during the day to nocturnal levels. On the basis of gene expression profiling and the levels of pathway intermediates, it is proposed that both increased metabolic flux and transcriptional activation of scent and colour genes underlie the enhancement of petunia flower colour and scent production by Pap1. The co-ordinated regulation of metabolic steps within or between pathways involved in vital plant functions, as shown here for two showy traits determining plant-pollinator interactions, provides a clear advantage for plantsurvival. The use of a regulatory factor that activates scent production creates a new biotechnological strategy for the metabolic architecture of fragrance, leading to the creation of novel genetic variability for breeding purposes.