Spatial-temporal expression of NDRG2 in brain tissues in a rat model of intracerebral hemorrhage: A pilot study
Spatial-temporal expression of NDRG2 in brain tissues in a rat model of intracerebral hemorrhage: A pilot study
复制标题
脑出血大鼠模型脑组织中NDRG2的时空表达：初步研究
DOI：
10.1016/j.neulet.2017.10.021
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发表时间：
2018-01-01
影响因子：
2.5
通讯作者：
Chen, Gang
中科院分区：
文献类型：
--
作者：
Gao, Lingfeng;Li, Xiang;Chen, Gang
N-myc下游调节基因2（NDRG 2）是N-myc下调基因家族的成员，属于α/β水解酶超家族，在细胞死亡中起重要作用。NDRG 2在脑出血后脑组织中的表达及其作用尚不清楚。在这项研究中，我们研究了NDRG 2在脑出血大鼠模型脑组织中的时空表达。采用Western blot法检测脑出血后3 h、6 h、12 h、24 h、48 h、72 h、7 d NDRG 2的表达水平。结果显示，脑出血后NDRG 2表达水平升高，24 h达高峰，随后下降。同时，我们还通过NeuN和GFAP（分别为神经元和星形胶质细胞的生物标志物）的免疫荧光分析来检测NDRG 2在脑组织中的细胞定位。结果表明，脑出血后NDRG 2主要表达于星形胶质细胞，而非神经元。此外，双染色结果显示，脑出血后大鼠脑组织中NDRG 2和TUNEL阳性细胞率显著升高。NDRG 2在脑出血中的作用有待进一步研究，抑制NDRG 2的表达可能对脑出血有潜在的治疗作用。
N-myc downstream regulated gene 2 (NDRG2) was a member of the N-myc down regulated gene family which belongs to the alpha/beta hydrolase superfamily and played important roles in cell death. To date, the expression and effects of NDRG2 in brain after intracerebral hemorrhage (ICH) are unclear. In this study, we investigated the spatial-temporal expression of NDRG2 in brain tissues in a rat model of ICH. The expression levels of NDRG2 were tested in 3 h, 6 h, 12 h, 24 h, 48 h, 72 h, and 7d after ICH by western blot analysis. The results showed that the NDRG2 levels were increased and peaked at 24 h after ICH, and then declined subsequently. Meanwhile, we also examined the NDRG2 cellular localization in brain tissues by immunofluorescence analysis with NeuN and GFAP (biomarker of neuron and astrocytes respectively). The results demonstrated that NDRG2 was mainly expressed in astrocytes, but not neurons, after ICH. Additionally, the results of double staining indicated that the rate of NDRG2- and TUNEL - positive cells was significantly higher in the brain tissues in rats after ICH. The roles of NDRG2 in ICH needed further investigation and inhibiting the expression of NDRG2 may have potential therapeutic effects in ICH.