Proteases and nucleases involved in the biphasic digestion process of the brown marmorated stink bug, Halyomorpha halys (Hemiptera: Pentatomidae)
Proteases and nucleases involved in the biphasic digestion process of the brown marmorated stink bug, Halyomorpha halys (Hemiptera: Pentatomidae)
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DOI：
10.1002/arch.21459
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发表时间：
2018-07-01
影响因子：
2.2
通讯作者：
Bonning, Bryony C.
中科院分区：
文献类型：
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作者：
Lomate, Purushottam R.;Bonning, Bryony C.
褐纹蝽（Halyomorpha halys）（半翅目：蝽科）是美国的一种入侵性农业害虫，其管理面临着巨大的挑战。这种多食性昆虫使用口外和肠道消化来阻止基于蛋白质或核苷酸的控制策略。本研究的目的是对来自 H. halys 唾液、唾液腺和肠道的消化酶（蛋白酶和核酸酶）进行生化表征。两种组织和唾液的酶谱截然不同：肠道中蛋白酶的最佳 pH 值为 6，其中半胱氨酸蛋白酶占主导地位。相比之下，唾液腺 (8-10) 和唾液 (7) 中蛋白酶活性的最佳碱性 pH 值反映了丰富的丝氨酸蛋白酶和组织蛋白酶活性。唾液中的 RNase 酶最为丰富，而唾液腺和唾液中的 dsRNase 和 DNase 活性高于肠道中的活性。这些截然不同的酶谱突显了这种入侵物种用于有效处理植物营养物质的双相消化系统。了解 H. halys 消化生理学知识将有助于采取针对消化酶的反制措施，或针对这种害虫采取基于蛋白质或核苷酸的管理方案进行适当保护。
Management of the brown marmorated stink bug, Halyomorpha halys (Hemiptera: Pentatomidae), an invasive, agricultural pest in the United States, has presented significant challenges. This polyphagous insect uses both extra-oral and gut-based digestion thwarting protein- or nucleotide-based control strategies. The objective of this study was to biochemically characterize the digestive enzymes (proteases and nucleases) from the saliva, salivary gland and the gut of H. halys. Enzyme profiles for the two tissues and saliva radically differ: The pH optimum for proteases in the gut was six, with cysteine proteases predominant. In contrast, the alkaline pH optima for protease activity in the salivary gland (8-10) and saliva (7) reflected abundant serine protease and cathepsin activities. RNase enzymes were most abundant in saliva, while dsRNase and DNase activities were higher in the salivary gland and saliva compared to those in the gut. These very different enzyme profiles highlight the biphasic digestive system used by this invasive species for efficient processing of plant nutrients. Knowledge of H. halys digestive physiology will allow for counteractive measures targeting digestive enzymes or for appropriate protection of protein- or nucleotide-based management options targeting this pest.