Feedback control of morphogenesis in fungi by aromatic alcohols
Feedback control of morphogenesis in fungi by aromatic alcohols
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DOI：
10.1101/gad.1411806
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发表时间：
2006-05-01
影响因子：
10.5
通讯作者：
Fink, GR
中科院分区：
文献类型：
--
作者：
Chen, H;Fink, GR
许多真菌响应环境线索而经历从单细胞酵母形式到侵入性丝状形式的发育转变。在这里，我们描述了一个群体信号通路，连接环境感知的形态发生在酿酒酵母。酵母细胞分泌芳香醇，其通过Tpk2p依赖性机制诱导FLO11的表达来刺激形态发生。这些醇的合成缺陷的突变体显示出减少的丝状生长，这是部分抑制这些芳香族醇的加入。这些自身信号醇的产生受氮调节：高氨通过抑制其生物合成途径的表达来限制它，而氮缺乏条件则激活它。此外，这些芳香醇的产生受细胞密度控制并受到正反馈调节，这需要转录因子Aro80 p。这些相互作用定义了一个群体感应电路，允许酵母菌对细胞密度和环境的营养状态做出反应。这些相同的自动调节分子不引起白色念珠菌的形态开关，这表明这些分子信号是种特异性的。
Many fungi undergo a developmental transition from a unicellular yeast form to an invasive filamentous form in response to environmental cues. Here we describe a quorum signaling pathway that links environmental sensing to morphogenesis in Saccharomyces cerevisiae. Saccharomyces cells secrete aromatic alcohols that stimulate morphogenesis by inducing the expression of FLO11 through a Tpk2p-dependent mechanism. Mutants defective in synthesis of these alcohols show reduced filamentous growth, which is partially suppressed by the addition of these aromatic alcohols. The production of these autosignaling alcohols is regulated by nitrogen: High ammonia restricts it by repressing the expression of their biosynthetic pathway, whereas nitrogen-poor conditions activate it. Moreover, the production of these aromatic alcohols is controlled by cell density and subjected to positive feedback regulation, which requires the transcription factor Aro80p. These interactions define a quorum-sensing circuit that allows Saccharomyces to respond to both cell density and the nutritional state of the environment. These same autoregulatory molecules do not evoke the morphological switch in Candida albicans, suggesting that these molecular signals are species-specific.