A core-skirt designed artificial cornea with orthogonal microfiber grid scaffold
A core-skirt designed artificial cornea with orthogonal microfiber grid scaffold
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DOI：
10.1016/j.exer.2020.108037
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发表时间：
2020-06-01
影响因子：
3.4
通讯作者：
Mi, Shengli
中科院分区：
文献类型：
--
作者：
Wang, Jieqiong;Chen, Yun;Mi, Shengli
人工角膜是治疗严重角膜缺损的有效选择。在本研究中，我们制备了具有正交微纤维网格支架的芯裙设计的人工角膜。我们通过直写技术制备了PCL正交微纤维网格支架，然后将其与压缩胶原蛋白（CC）结合以获得三明治状的CC/P（其中P代表PCL微纤维网格支架）。 PHEMA 水凝胶和 CC/P 分别充当核心和裙边，P 还充当两者之间的中间体。对人工角膜的物理性能进行了评价，包括形态、力学性能和透光率。 SEM图像显示核心和裙部之间的界面处有效连接且缺乏相分离，并且裙部形成了高度多孔的支架，促进了组织生物整合。此外，我们使用裙子结构构建了包含两种细胞类型的角膜组织模型：角膜基质干细胞（CSSC）和小鼠海马神经元。结果表明，细胞能够良好生长和分化，正交微纤维网格支架纤维对CSSC和神经元轴突的结构生长有良好的引导作用。
Artificial cornea is an effective treatment option for cases of severe corneal loss. In this study, we prepared a core-skirt designed artificial cornea with orthogonal microfiber grid scaffold. We fabricated PCL orthogonal microfiber grid scaffolds by a direct writing technique, and then combined them with compressed collagen (CC) to obtain a sandwich-like CC/P (where P is used to represent the PCL microfiber grid scaffold). PHEMA hydrogel and the CC/P served as the core and the skirt, respectively, with the P also serving as an intermediate between the two. The physical properties of the artificial cornea, including the morphology, the mechanical properties and the light transmittance, were evaluated. SEM images showed an effective connection and a lack of phase separation at the interface between the core and the skirt, and the skirt formed a highly porous scaffold that promoted tissue biointegration. In addition, we used the skirt structure to construct a corneal tissue model containing two cells types: corneal stromal stem cells (CSSCs) and mouse hippocampal neurons. The results showed that the cells could grow and differentiate well, and the orthogonal microfiber grid scaffold fibers were good guides for the structural growth of CSSCs and neuronal axons.