Effect of Corticotropin‐Releasing Hormone Antagonist on Oestrogen‐Dependent Glucoprivic Suppression of Luteinizing Hormone Secretion in Female Rats
Effect of Corticotropin‐Releasing Hormone Antagonist on Oestrogen‐Dependent Glucoprivic Suppression of Luteinizing Hormone Secretion in Female Rats
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促肾上腺皮质激素释放激素拮抗剂对雌性大鼠雌激素依赖性促黄体激素分泌的抑制作用
DOI：
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发表时间：
1999
影响因子：
3.2
通讯作者：
K. Maeda
中科院分区：
文献类型：
--
作者：
S. Tsukahara;H. Tsukamura;D. L. Foster;K. Maeda
2-脱氧葡萄糖（2DG）药理学降低葡萄糖可用性可抑制大鼠和生长迟缓羔羊的脉冲式促黄体生成素（LH）分泌。性腺类固醇增强大鼠LH分泌的葡萄糖抑制。本研究确定促肾上腺皮质激素释放激素（CRH）是否在介导LH分泌的雌激素依赖性和非依赖性葡萄糖抑制中发挥作用。本研究在卵巢切除（OVX）大鼠中进行，其中一些大鼠接受了含有溶于花生油的雌二醇-17 β（OE 2）（20 μg/ml）的硅橡胶植入体，以产生生理血浆水平的OE 2（30 pg/ml）。 卵巢切除术后7天，将引导套管立体定位植入大鼠第三脑室。7天后，通过留置的心房插管每6分钟采集一次血液样品，持续3 h用于LH脉冲测定。 采血后第1小时，在通过留置心房插管给予2DG前，通过植入的插管向第三脑室注射CRH拮抗剂[d-Phe 12，Nle 21，38]hCRF-（21-41）或溶剂。2DG（200 mg/kg b.w.） 在携带OE 2植入物的溶剂处理大鼠中。CRH拮抗剂（5.65 nmol）阻断了2DG对OE 2处理的OVX动物中脉冲式LH分泌的抑制作用。 另一方面，在不存在雌激素的情况下，两倍大剂量的2DG（400 mg/kg b.w.） 5倍量的CRH拮抗剂（28.3nmol）不能阻断。 这些结果表明，介导葡萄糖依赖性LH分泌抑制的机制涉及两种成分：一种是雌激素依赖性，另一种是雌激素非依赖性。CRH可能参与LH分泌葡萄糖抑制的雌激素依赖性成分，但不是雌激素非依赖性成分。
Pharmacological reduction of glucose availability with 2‐deoxyglucose (2DG) suppresses pulsatile luteinizing hormone (LH) secretion in rats and growth‐retarded lambs. Gonadal steroids enhance the glucoprivic suppression of LH secretion in rats. The present study determined if corticotropin‐releasing hormone (CRH) plays a role in mediating oestrogen‐dependent and ‐independent glucoprivic suppression of LH secretion. The study was conducted in ovariectomized (OVX) rats some of which received Silastic implants containing oestradiol‐17β (OE2) dissolved in peanut oil at 20 μg/ml to produce a physiological plasma level of OE2 (30 pg/ml). Seven days after ovariectomy, the rats were stereotaxically implanted with a guide cannula into the third cerebral ventricle. Seven days later, blood samples were collected through an indwelling atrial cannula every 6 min for 3 h for LH pulse determination. After the first hour of blood sampling, a CRH antagonist, [ d‐Phe12, Nle21,38]hCRF‐(21–41), or vehicle was injected into the third cerebral ventricle through the implanted cannula before 2DG administration through the indwelling atrial cannula. Pulsatile LH secretion was suppressed by 2DG (200 mg/kg b.w.) in the vehicle‐treated rats bearing OE2 implants. The CRH antagonist (5.65 nmol) blocked the suppressive effect of 2DG on pulsatile LH secretion in the OE2‐treated OVX animals. On the other hand, in the absence of oestrogen, the effect of a twice greater dose of 2DG (400 mg/kg b.w.) was not blocked by five times greater amount of CRH antagonist (28.3 nmol). These results suggest the mechanisms mediating glucoprivic suppression of LH secretion involve two components: one is oestrogen‐dependent and the other oestrogen‐independent. CRH may be involved in the oestrogen‐dependent component of glucoprivic suppression of LH secretion but not the oestrogen‐independent one.
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DOI：
10.1210/endo.137.2.8593808
发表时间：
1996
期刊：
Endocrinology.
影响因子：
--
作者：
Bucholtz,DC;Vidwans,NM;Herbosa,CG;Schillo,KK;Foster,DL
通讯作者：
Foster,DL
葡萄糖可用性的减少抑制雌性和雄性大鼠的脉动黄体生成激素释放。
DOI：
10.1210/endo.137.4.8625885
发表时间：
1996
期刊：
Endocrinology.
影响因子：
--
作者：
Nagatani,S;Bucholtz,DC;Murahashi,K;Estacio,MA;Tsukamura,H;Foster,DL;Maeda,KI
通讯作者：
Maeda,KI
通过限制葡萄糖可用性来抑制黄体生成素脉冲是由脑干中的传感器介导的。
DOI：
10.1210/endo.137.4.8625886
发表时间：
1996
期刊：
Endocrinology.
影响因子：
--
作者：
Murahashi,K;Bucholtz,DC;Nagatani,S;Tsukahara,S;Tsukamura,H;Foster,DL;Maeda,KI
通讯作者：
Maeda,KI
室旁去甲肾上腺素释放介导糖皮质激素对脉动黄体生成素分泌的抑制。
DOI：
10.1210/endo.137.8.8754737
发表时间：
1996
期刊：
Endocrinology.
影响因子：
--
作者：
Nagatani,S;Tsukamura,H;Murahashi,K;Bucholtz,DC;Foster,DL;Maeda,K
通讯作者：
Maeda,K