Lrriq1 is an essential factor for fertility by suppressing apoptosis
Lrriq1 is an essential factor for fertility by suppressing apoptosis
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DOI：
10.1007/s10815-022-02623-9
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发表时间：
2022-09
影响因子：
3.1
通讯作者：
Mayu Fukutomi;Chiharu Uedono;A. Fujii;Youichi Sato
中科院分区：
文献类型：
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作者：
Mayu Fukutomi;Chiharu Uedono;A. Fujii;Youichi Sato
目的研究亮氨酸重复序列和IQ基序1（LRRIQ 1）基因与血浆白蛋白B水平的关系。然而，LRRIQ 1的功能仍不清楚。在这项研究中，我们产生了Lrriq 1基因敲除小鼠（Lrriq 1 −/−小鼠），并研究了LRRIQ 1对BMP 2 B和生育力的影响。抑制素B的表达使用从3月龄雄性小鼠的睾丸提取的蛋白质通过蛋白质印迹法检测。使用7周龄Lrriq 1 −/−小鼠和野生型（WT）小鼠进行交配实验，以检查生育力。使用3个月大的雄性小鼠的精子浓度和精子活力进行了测定。B表明，Lrriq 1 −/−小鼠的精子结合素α（Inha）的mRNA和蛋白水平降低，这构成了α亚基。在交配实验中，Lrriq 1 −/−雄性小鼠的窝仔数为4.3 ± 2.9，显著低于WT雄性小鼠的窝仔数（8.3 ± 1.3）（p< 0.001）。在Lrriq 1 −/−和WT雄性小鼠之间未观察到精子计数差异;然而，与WT小鼠（70.2 ± 4.7）相比，Lrriq 1 −/−小鼠（48.4 ± 4.9）的精子活力（%）显著降低（p< 0.001）。结论Lrriq 1基因敲除小鼠睾丸生殖细胞和附睾精子凋亡，导致精子活力降低，产羔数减少。
PurposeLeucine-rich repeats and IQ motif containing 1 (LRRIQ1) gene is reportedly associated with plasma inhibin B levels. However, the function ofLRRIQ1remains unknown. In this study, we generatedLrriq1knockout mice (Lrriq1−/−mice) and examined the effects ofLRRIQ1on inhibin B and fertility.MethodsLrriq1−/−mice were generated using CRISPR/Cas9 genome editing technology. The expression of Inhibin B was examined by Western blotting using a protein extracted from the testis of a 3-month-old male mouse. Mating experiments were conducted using 7-week-oldLrriq1−/−mice and wild-type (WT) mice to examine fertility. Sperm concentration and sperm motility were measured using 3-month-old male mice.ResultsExpression analysis of inhibin B revealed thatLrriq1−/−mice exhibited reduced mRNA and protein levels of inhibin alpha (Inha), which constitutes the α subunit. In the mating experiment, the litter size ofLrriq1−/−male mice was 4.3 ± 2.9, which was significantly lower than that of WT male mice (8.3 ± 1.3) (p< 0.001). No difference in sperm count was observed betweenLrriq1−/−and WT male mice; however, sperm motility (%) was significantly reduced inLrriq1−/−mice (48.4 ± 4.9) when compared with WT mice (70.2 ± 4.7) (p< 0.001). Based on TUNEL staining, the testes and epididymal sperm ofLrriq1−/−mice showed high numbers of apoptosis-positive cells.ConclusionLrriq1knockout reduced sperm motility and litter size by inducing apoptosis of testicular germ cells and epididymal sperm.