EphB4 expression in pterygium is associated with microvessel density.
EphB4 expression in pterygium is associated with microvessel density.
复制标题
DOI：
--
复制
发表时间：
2014-11
影响因子：
0.1
通讯作者：
C. Xue;Yueqin Chen;Zhenping Huang;Yirui Ge;Haiyan Wang;Jiandong Wang
中科院分区：
文献类型：
--
作者：
C. Xue;Yueqin Chen;Zhenping Huang;Yirui Ge;Haiyan Wang;Jiandong Wang
目的：血管生成是翼状胬肉发病过程中的一个重要环节，是角膜上血管纤维组织的生长。EphB 4是ephrin-Eph系统的受体酪氨酸激酶，在胚胎发育和肿瘤血管生成过程中起重要作用，并可能参与眼部血管生成。本研究的目的是探讨EphB 4在翼状胬肉中的作用。方法采用免疫组织化学方法检测15例翼状胬肉及其配对的上球结膜组织中EphB 4和CD 31的表达。采用实时荧光定量RT-PCR方法检测7例翼状胬肉及相应的上球结膜组织中EphB 4基因mRNA的表达水平。用靶向CD 31的抗体评估微血管密度（MVD）。结果EphB 4蛋白在翼状胬肉上皮和间质中的表达高于球结膜上皮和间质。免疫组化染色显示翼状胬肉结膜微血管平均计数高于正常结膜（28.24 ± 6.79个/高倍视野比11.09 ± 2.96个/高倍视野，P < 0.001）。翼状胬肉及正常结膜间质MVD值与EphB 4染色呈显著相关（P < 0.001）。与自体上球结膜移植物相比，在4对翼状胬肉中EphB 4 mRNA的表达增加，包括3例复发性翼状胬肉。结论翼状胬肉组织中EphB 4的表达与MVD的增加密切相关，可能参与了翼状胬肉组织中血管生成的过程。EphB 4蛋白是治疗翼状胬肉的潜在靶点。
OBJECTIVE Angiogenesis is a key component of the pathogenesis process of pterygium, which is the growth of fibrovascular tissue on the cornea. It has been reported that EphB4, a receptor tyrosine kinase of the ephrin-Eph system, plays important role in vascular development during embryogenesis and tumor angiogenesis and is potentially involved in ocular angiogenesis. The aim of this study is to investigate the role of EphB4 in pterygia. METHODS Fifteen pterygium samples and their paired upper bulbar conjunctiva were evaluated for expression of EphB4 and CD31 by using immunohistochemical staining. The expression level of the mRNA of EphB4 gene in 7 pterygia and matched upper bulbar conjunctiva was evaluated by using a quantitative real-time RT-PCR analysis. Microvessel density (MVD) was assessed with antibody that targets CD31. RESULTS EphB4 protein was high expressed in the epithelium and stroma of pterygia compared to those in upper bulbar conjunctiva. Immunohistochemical staining showed that pterygia presented with statistically significant higher average count of microvessel compared to normal conjunctivae (28.24 ± 6.79 vs 11.09 ± 2.96 per high power field, P < 0.001). MVD values in stroma of the pterygium and normal conjunctiva presented a significant correlation with EphB4 staining (P < 0.001). Compared with autologous upper bulbar conjunctiva grafts, the expression of the EphB4 mRNA was increased in pterygia in 4 paired samples, including those 3 recurrent ones. CONCLUSION The expression of EphB4 in pterygium was significantly related with the increased MVD and may be involved in angiogenesis. EphB4 protein is a potential target for treatment of pterygium.